Cytokines Genes Polymorphisms in Ukrainian HIV-1 Infected Individuals

P37.07

Background: The objective of the research was to study distribution character of the allelic variants of cytokines genes in HIV-1 infected Ukrainians.

Methods: Data for the study were DNA samples, received from 200 inhabitants of Ukraine: 78 HIV-infected, 22 - HIV-negative individuals from the group of high risk of contamination, 100 healthy blood donors. IL-4 (−590C/T), IL-10 (−592C/A) and TNF-α (−308G/A) genes polymorphisms detection was made with PCR-RLFP method.

Results: By analysis of frequency of IL-4 gene allelic variants it has been discovered that homozygotes by the main allel were the dominant variant. Among people with HIV T/T minor gene carriers were 4.5 more often met in comparison with control group (p<0.05) that can prove the tendency to association of the mentioned genotype with infection.

Distribution of allelic variants of IL-10 gene promoter region in position−592 is characterized by homozygote dominance by the main gene. Among the individuals with HIV A/A minor allel carriers were 3.4 more often met in comparison with control group (p<0.05). Individuals with A/A genotype were not identified in group of high risk of virus infection. The abovementioned proves the tendency to association of minor allel carrier state with HIV infection.

The occurrence of the homozygous combination of the allelic variant G/G of the promoter of TNF-α has been shown to prevail almost twofold over the occurrence of the variant G/A among all groups. High frequency of heterozygote by the main allel has been recorded among the individuals with HIV. Thus, G/A genotype frequency in group of HIV-infected people 2 and 1.5 exceeded the appropriate indices of group of high risk of infection and comparison group correspondingly (p<0.05) that points to the tendency to association of the mentioned variant with infection.

Conclusions: Cytokines genes variations may contribute to the acquisition of HIV infection and encourages carrying out of further populations studies in this sphere of HIV-infection immunogenetics.