Ultrastructural changes in vascular endothelium after an intra-aortic in jection of 65% meglumine diatrizoate, 10 ml/kg, and equiosmolal hypertonic saline were observed using electron microscopy in the rat. Cell shrinkage and expansion of intercellular clefts were found in all specimens from a large artery at 5 and 20 minutes after injection. Some destroyed cells were seen at all times after contrast and hypertonic saline administration, but one-half of the specimens taken at 1 hour were otherwise nearly normal in appearance. In muscle capillaries, the changes were less severe than in the large artery. Despite the large dose, equiosmolal meglumine diatrizoate and saline solu tions produced identical vascular lesions as detected with electron micros copy.
References
1.
Winzer, K., Langecker, H., Junkmann, K.: Zur Frage der Verträglichkeit von Nierenund Gallekontrastmitteln. Ärztl. Wochenschr., 9: 950, 1954.
2.
McAlister, W.H. , Shackelford, G.D., Kissane, J.: The histological effects of 30% Cystokon, Hypague 25%, and Renografin-30 in the bladder. Radiology, 104: 563, 1972.
3.
Praestholm, J., Ølgaard, K.: Comparative histological investigation of the sequelae of experimental myelography using sodium methiodal and meglumine iothalamate. Neuroradiology, 4: 14, 1972.
4.
Sörensen, S.E. : Local toxic effects of anaesthetics and contrast media in urethrography. Acta Radiol. (Diagn.) (Stockh.) , 12: 225, 1972.
5.
Lea-Thomas, M. : Gangrene following peripheral phlebography of the legs. Br. J. Radiol., 43: 528, 1970.
6.
Göthlin, J., Hallböök, T.: Skin necrosis following extravasal injection of contrast medium in phlebography . Radiologe, 11: 161, 1971.
7.
Spigos, D.G., Thane, T.T., Capek, V.: Skin necrosis following extravasation during peripheral phlebography. Radiology, 123: 605, 1977.
8.
Leung, P.C., Cheng, C.Y.: Extensive local necrosis following the intravenous use of X-ray contrast medium in the upper extremity. Br. J. Radiol., 53: 361, 1980.
9.
Gonsette, R. , Andre-Balisaux, G.: La perméabilité des vaissaux cérébraux. Étude systématique de la tolérance des capillaires cérébraux pour les produits de contraste utilisés en artériographie. Acta Radiol. Suppl., 270: 228, 1967.
10.
Moss, A.A., Margulis, A.R., Youker, J.E., et al.: Effect of intra-arterial contrast media on the small intestine of rats. A preliminary report of an electron microscope study. Invest. Radiol., 6: 392, 1971.
11.
Moss, A.A., Margulis, A.R., Lee, J.C., et al.: The effect of intra-arterial contrast media on the small intestine of rabbits. Radiology, 108: 279, 1973.
12.
Ritchie, W.G.M. , Lynch, P.R., Stewart, G.J.: The effect of contrast media on normal and inflamed canine veins. A scanning and transmission electron microscopic study. Invest. Radiol., 9: 444, 1974.
Waldron, R.L. , II., Bridenbaugh , R., Purkerson, M., et al.: The effect of angiographic contrast media at the cellular level in the brain. Radiology, 108: 187, 1973.
15.
Waldron, R.L. , II., Bridenbaugh , R.B., Dempsey, E.W.: Effect of angiographic contrast media at the cellular level in the brain: Hypertonic vs. chemical action. Am. J. Roentgen., 122: 469, 1974 .
16.
Waldron, R.L. , II., Bridenbaugh , R.B., Dempsey, E.W.: Alterations at the cellular level in the brain following repeated angiography: Protective effect of corticosteroids. J. Assoc. Can. Radiol.26: 82, 1975.
17.
Waldron, R.L. , II., Bryan, R.N.: Effects of contrast agents on the blood-brain barrier. An electron microscopic study. Radiology, 116: 195, 1975.
18.
Sterrett, P.R. , Bradley, I.M., Kitten, G.T., et al.: Cerebrovasculature permeability changes following experimental cerebral angiography. A light- and electron-microscopic study. J. Neurol. Sci., 30: 385, 1976.
19.
Casady, R.L. , Kitten, G.T., Bradley, I.M., et al.: Sites of cerebrovascular injury induced by radiographic contrast media. Am. J. Anat., 153: 477, 1978.
20.
Brightman, M.W., Reese, T.S.: Junctions between intimately apposed cell membranes in the vertebrate brain. J. Cell Biol., 40: 648, 1969.
21.
Sterrett, P.R. , Thompson, A.M., Chapman, A.L., et al.: The effects of hyperosmolality on the blood-brain barrier. A morphological and physiological correlation . Brain Res., 77: 281, 1974.
22.
Shimamoto, T.: Panel Discussion. Angiology , 25: 708, 1974.
23.
Constantinides, P., Robinson, M.: Ultrastructural injury of arterial endothelium. I. Effects of pH, osmolarity, anoxia, and temperature. Arch. Pathol., 88: 99, 1969.
24.
Mann, S., Zeitler, E.: Verhalten der Serumosmolarität bei hohen Kontrastmitteldosen im Rahmen der Angiographie. Fortschr. Roentgenstr. Nucl. Med., 122: 135, 1975.
25.
Lindgren, I. , Virtama, P.: Wirkungen der Temperature des Kontrastmittels bei intra-arteriellen Injektionen. Eine vitalmikroskopische Studie. Roentgenblaetter, 21: 1, 1968.
26.
Wiedeman, M.P. : Vascular and intravascular responses to various contrast media. Angiology, 14: 107, 1963.
27.
Sutherland, G.R., Räsänen, O.: The effects of intra-arterial injections of radiographic contrast media on the mesenteric circulation in rats. Clin. Radiol., 18: 200, 1967.
