An investigation was conducted on the effect of formocresol treatment on the aqueous extractability and enzymatic susceptibility of excised implant tissue. The treated tissue demonstrated a decreased solubility and a diminished digestibility by trypsin, pepsin and collagenase. However, its reactivity toward hyaluronidase showed little alteration.
References
1.
Buckley, J.P. : The Chemistry of Pulp Decomposition with a Rational Treatment for This Condition and Its Sequelae, Am Dent J3:764-771, 1904.
2.
's- Gravenmade, E.J.: Some Biochemical Considerations of Fixation in Endodontics, J Endodontics1:233-237, 1975.
3.
French, D., and Edsall, J.T.: The Reaction of Formaldehyde with Amino Acids and Proteins, in Advances in Protein Chemistry, Vol. 2. Anson, M.L. and Edsall, J.T. (eds.) New York, Academic Press, pp 227-335, 1945.
4.
Bowes, J.H., and Cater, C.W.: Cross-Linking of Collagen, J Appl Chem15:296-304, 1965.
5.
Berger, J.E.: Pulp Tissue Reaction to Formocresol and Zinc Oxide-Eugenol, J Dent Child32:13-28, 1965.
6.
Boucek, R.J., and Noble, N.L.: Connective Tissue, A Technique for its Isolation and Study, AMA Arch of Path59:552-558, 1955.
7.
Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; and Smith, F.: Colorimetric Method for Determination of Sugars and Related Substances , Anal Chem28:350-356, 1956.
8.
Woessner, J.F.: The Determination of Hydroxyproline in Tissue and Protein Samples Containing Small Proportions of Imino Acids, Arch Biochem Biophys93:440-497, 1961.
9.
Fraenkel-Conrat, H., and Olcott, H.S.: The Reaction of Formaldehyde with Proteins. V. Cross-Linking between Amino and Primary Amide or Guanidyl Groups, J Am Chem Soc70:2673-2684, 1948 .
10.
Fraenkel-Conrat, H., and Olcott, H.S.: Reaction of Formaldehyde with Proteins. VI. Cross-Linking of Amino Groups with Phenol, Imidazole, or Indole Groups, J Biol Chem:174:827-843, 1948.
11.
Fraenkel-Conrat, H., and Mecham, D.K.: The Reaction of Formaldehyde with Proteins. VII. Demonstration of Intermolecular Cross-Linking by Means of Osmotic Pressure Measurements, J Biol Chem177:477-486, 1949.
12.
Harris, E.D. JR., and Farrell , M.E.: Resistance to Collagenase: A Characteristic of Collagen Fibrils Cross-Linked by Formaldehyde, Biochem Biophys Acta278:133-141, 1972.
13.
Martin, C.J. , and Marini, M.A.: Spectral Detection of the Reaction of Formaldehyde with the Histidine Residues of Chymotrypsin, J Biol Chem242:5736-5743, 1967.
14.
Feldman, M. Ya.: Reactions of Nucleic Acids and Nucleoproteins with Formaldehyde, in Progress in Nucleic Acid Research and Molecular Biology, Davidson, J.N. and CoHn, W.E. (eds.), 13:1-49, 1973.
15.
Walker, J.F. : Formaldehyde, 3d ed. Rein-hold, New York, 1964.
16.
Birkedal-Hansen, H.: Effect of Fixation on Detection of Carbohydrates in Demineralized Paraffin Sections of the Rat Jaw, Scand J Dent Res82:99-112, 1974.
