A family of acidic proteins has been isolated from human submandibular-sublingual saliva using Sephadex G-200 gel filtration, DE-52 cellulose chromatography, and immuno-affinity chromatography. These molecules were homogenous in size and by immunological criteria, but exhibited charge heterogeneity. The heterogeneity displayed was not due to gustatory stimulation or degradation during isolation procedures.
References
1.
Kleinberg, I.; Ellison, S.A.; and Mandel, I.D.: Saliva and Dental Caries, Special Supplement of Microbial Abstracts , New York and London: Information Retrieval, Inc., 1979.
2.
Cowman, R.A. ; Schaefer, S.J.; and Fitzgerald , R.J.: Specificity of Utilization of Human Salivary Proteins for Growth by Oral Streptococci , Caries Res13:181-189, 1979.
3.
Cowman, R.A. ; Schaefer, S.J.; Fitzgerald , R.J.; Rosner, D.; Shklair, I.L.; and Walter, R.G.; Differential Utilization of Proteins in Saliva from Caries-active and Caries-free Subjects as Growth Substrates by Plaque-forming Streptococci, JDent Res58:2019-2027, 1979.
4.
Choih, S.J.; Smith, Q.T.; and Schachtele, C.F. : Modification of Human Parotid Saliva Proteins by Oral Streptococcus sanguis, J Dent Res58:516-523, 1979.
5.
Mayo, J.W. and Carlson, D.M.: Protein Composition of Human Submandibular Secretions, Arch Biochem Biophys161:134-145,1974.
6.
Sturman, I.M. ; Stevens, R.H.; Dipaola , C.; Tabak, L.; and Mandel, I.D.: Partial Characterization of Most Anionic Submaxillary Glycoprotein, IADR Progr & Abst54:No. 264, 1975.
Masson, P.L. ; Carbonara, A.O.; and Heremans , J.F.: Studies on the Proteins of Human Saliva, Biochim Biophys Acta107:485-500, 1965.
9.
Gugler, E.; Pallavicini, J.C.; Swerdlow, H.; Zipkin, I.; and Di Sant'Agnese, P.A.: Immunological Studies of Submaxillary Saliva from Patients with Cystic Fibrosis and from Normal Children, J Pediatr73:548-559, 1968.
10.
Juriaanse, A.C. and Booij, M.: Isolation and Partial Characterization of Three Acidic Proteins from Human Submandibular Saliva, Arch Oral Biol24:621-625, 1979.
11.
Juriaanse, A.C. and Booij, M.: Isolation and Characterization of the Main Neutral Protein from Human Submandibular Saliva, Arch Oral Biol24:825-828, 1979.
12.
Levine, M.J. ; Herzberg, M.C.; Levine , M.S.; Ellison, S.A.; Stinson, M.W.; Li , H.C.; and Van Dyke , T.: Specificity of Salivary-Bacterial Interactions: Role of Terminal Sialic Acid Residues in the Interaction of Salivary Glycoproteins with Streptococcus sanguis and Streptococcus mutans , Infect Immun19:107-115, 1978.
13.
Lowry, O.H. ; Rosebrough, N.J.; Farr , A.L.; and Ran-Dall , R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
14.
Roe, J.H. : The Determination of Sugar in Blood and Spinal Fluid with Anthrone Reagent , J Biol Chem212:335-343, 1955.
15.
Lowry, O.H. ; Roberts, N.R.; Leiner , K.Y.; Wu , M.L.; and Farr, A.L.: The Quantitative Histochemistry of Brain . I. Chemical Methods, J Biol Chem207:1-17, 1954.
16.
March, S.C. ; Parikh, I.; and Cuatrecasas , P.: A Simplified Method for Cyanogen Bromide Activation of Agarose for Affinity Chromatography , Anal Biochem60:149-152, 1974.
17.
Fairbanks, G.; Steck, T.L.; and Wallach, D.F.H.: Electrophoretic Analysis of the Major Polypeptides of the Human Erythrocyte Membrane, Biochemistry10:2606-2617, 1971 .
18.
Ornstein, L. : Disc Electrophoresis. I. Background and Theory , Ann NY Acad Sci121:321-349, 1964.
19.
Davis, B.J. : Part II. Clinical Applications Disc Electrophoresis. II. Method and Application to Human Serum Proteins, Ann NY Acad Sci121:404-427, 1964.
20.
Bernfeld, P.: Amylases, a and β, Methods in Enzymology, Vol 1, Colowick, S.P. and Kaplan, N.O., Eds., New York: Academic Press, 1955, pp. 149-158.
21.
Levine, M.J. and Ellison, S.A.: Immuno-electrophoretic and Chemical Analyses of Human Parotid Saliva, Arch Oral Biol18:839-853, 1973.
22.
Levine, M.J. ; Weill, J.C.; and Ellison, S.A.: The Isolation and Analysis of a Glycoprotein from Parotid Saliva, Biochim Biophys Acta188:165-167,1969.
23.
Li, H.C. and Levine, M.J.: Characterization of a Glycopeptide from the Proline-rich Glycoprotein of Human Parotid Saliva, Arch Oral Biol25:353-355, 1980.
24.
Cole, M.F.; Arnold, R.R.; Mestecky, J.; Prince, S.; Kulhavy, R.; and McGhee, J.R. : Microbial Aspects of Dental Caries, Stiles, H.M., Loesche, W.J., and O'Brien, T.C., Eds., Spec. Suppl. of Microbial Abstracts, New York and London: Information Retrieval, Inc., pp. 359-374, 1976.
25.
BRANDTZAEG, P.; FJELLANDER, I.; and GJERULDSEN, S.T.: Human Secretory Immunoglobulins. I. Salivary Secretions from Individuals with Normal or Low Levels of Serum Immunoglobulins, Scand J Haemat Suppl. 12:1-83, 1970. 26. HERZBERG, M.C.; LEVINE, M.J.; and TABAK, L.A.: Purifi
26.
cation and Characterization of Monkey Salivary Mucin, J Biol Chem254:1487-1494, 1979.
27.
OPPENHEIM, F.G.; HAY, D.I.; and FRANZBLAU, C.: Proline-rich Proteins from Human Parotid Saliva. I. Isolation and Partial Characterization, Biochemistry 10:4233-4238, 1971. 28. HENKIN, R.L.; LIPPOLDT, R.E.; BILSTAD, J.; WOLF, R.O.; LUM, C.K.L.; and EDELHOCH, H.: Fractionation of Human Parotid Saliva Proteins, J Biol Chem 253:7556-7565, 1978. 29. LEVINE, M.J.; ELLISON, S.A.; and BAHL, O.P.: The Isolation from Human Parotid Saliva and Partial Characterization of the Protein Core of a Major Parotid Glycoprotein, Arch Oral Biol 18:827-837, 1973.
28.
Kauffman, D.L. ; Watanabe, S.; and Keller, P.J.: Short Communications: The Relationship of Flow Rate to Glycosylation of Human Parotid Amylase, Arch Oral Bio10:597-599, 1974.
29.
Muenzer, J. ; Bildstein, C.; Gleason , M.; and Carlson, D.M.: Purification of Proline-rich Proteins from Parotid Glands of Isoproterenol-treated Rats , J Biol Chem254 :5623-5628, 1979 .
30.
Muenzer, J. ; Bildstein, C.; Gleason , M.; and Carlson, D.M.: Properties of Proline-rich Proteins from Parotid Glands of Isoproterenol-treated Rats , J Biol Chem254 :5629-5634,1979.
