Demonstration of Physiological Barrier Between Pulpal Odontoblasts and its Perturbation Following Routine Restorative Procedures: A Horseradish Peroxidase Tracing Study in the Rat
Available accessResearch articleFirst published online August, 1989
Demonstration of Physiological Barrier Between Pulpal Odontoblasts and its Perturbation Following Routine Restorative Procedures: A Horseradish Peroxidase Tracing Study in the Rat
Vascular injection of the macromolecular tracer, horseradish peroxidase (HRP), was used to study the permeability of the odontoblast cell layer in developing and mature rat molar teeth, and to investigate the effect of cavity preparations on the permeability of this epithelioid cell layer in adult animals. HRP injected into the vascular system of normal animals 28 days of age and older was localized histochemically (from 5 to 90 min after injection) throughout the extracellular spaces of the maxillary dental pulps; however, the tracer did not penetrate beyond the tight junctions at the apical region of the odontoblast cell layer, and was absent from the predentin and dentin. In contrast, HRP injected into very young neonatal animals (e.g., day 3) resulted in free passage of HRP between odontoblasts and into the overlying predentin and dentin. When Class V cavities had been prepared in adult maxillary molars after HRP was injected into the blood stream, HRP reaction product penetrated the predentin and dentin immediately beneath the cavity preparation; however, adjacent, un-traumatized areas of predentin and dentin in the operated teeth were devoid of reaction product. These results provide evidence that: (1) a physiological barrier develops between the distal segments of odontoblast cell bodies in normal rat molar teeth between days 15 and 28 of postnatal life, and this barrier prevents the passage of macromolecules from the pulp into the predentin and dentin; and (2) this barrier is perturbed following routine restorative procedures in adult animals.
References
1.
Adams, J.C. (1977): Technical Considerations on the Use of Horseradish Pcroxidasc as a Neuronal Marker, Neuroscience2:141-145.
2.
Arwill, T. (1967): Studies on the Ultrastructure of Dental Tissues , Odont Revy18:191-208.
3.
Arwill, T. (1968): The Ultrastructurc of the Pulpo-Dentinal Border Zone. In: Dentin and Pulp, N.B.B. Symons, Ed., Baltimorc : Williams and Wilkins Co., pp. 147-167.
4.
Bishop, M.A. (1985): Evidence for Tight Junctions Between Odontoblasts in the Rat Incisor, Cell Tissue Res239:137-140.
5.
Bishop, M.A. (1987): An Investigation of Pulp Capillaries and Tight Junctions Between Odontoblasts in Cats, Anat Embryol177:131-138.
6.
Brannstrom, M. (1963): Dentin Sensitivity and Aspiration of Odontoblasts , J Am Dent Assoc66:366-370.
7.
Brannstrom, M. and Astrom, A. (1964): A Study on the Mechanism of Pain Elicited from the Dentin, J Dent Res43:619-625.
8.
Byers, M.R. (1977): Fine Structure of Trigeminal Receptors in Rat Teeth. In: Pain in the Trigeminal Region, D.J. Anderson and B. Matthews , Eds., North Holland: Elsevier , pp. 13-24.
9.
Byers, M.R. (1979): Large and Small Trigeminal Nerve Endings and Their Associations With Odontoblasts in Rat Molar Dentin and Pulp, Adv Pain Res Ther3:265-270.
10.
Byers, M.R. (1984): Dentttl Sensory Receptors, Int Rev Neurobiol25 :39-93.
11.
Carson, K.A. and Mesulam, M.-M. (1982): Electron Microscopic Demonstration of Neural Connections Using Horseradish Peroxidase: a Comparison of the Tetramethylbenzidine Procedure with Seven Other Histochemical Methods, J Histochem Cytochem30:425-435.
12.
Calle, H. (1985): Intercellular Junctions Between Human Odontoblasts , Acta Anat122:138-144.
13.
Dermietzel, R.; Meller, K.; Tetzlaff, W.; and Waelsh, M. (1977): In vivo and in vitro Formation of the Junctional Complex in Choroid Epithclium. A Frcczc-ctching Study, Cell Tissue Res181 :427-441.
14.
Eda, S. and Saito, T. (1978): Electron Microscopy of Cells Displaced into the Dentinal Tubules due to Dry Cavity Preparation, J Oral Puthol7:326-335.
15.
Farquhar, M.G. and Palade, G.E. (1963): Junctional Complexes in Various Epithclia, J Cell Biol17:375-408.
16.
Frank, R.M. (1968): Ultrastructural Relationship Between the Odontoblast, its Process and the Nerve Fibre. In: Dentine and Pulp. Their Structure and Reactions, N.B.B. Symons , Ed., Baltimore: Williams and Wilkins Co., pp. 115-146.
17.
Garant, P.R.; Szabo, G.; and Nalbandian, J. (1968): The Fine Structure of the Mouse Odontoblast, Arch Oral Biol13:857-876.
18.
Graham, R.C. and Karnovsky, M.J. (1966): The Early Stages of Absorption of Injected Horseradish Peroxidase in the Proximal Tubule of Mouse Kidney: Ultrastructural Cytochemistry by a New Technique, J Histochem Cytochem14:291-302.
19.
Goodenough, D.A. and Revel, J.P. (1970): A Fine Structural Analysis of Intercellular Junctions in the Mouse Liver, J Cell Biol45:272-290.
20.
Grynszpan-Wynograd, O. and Nicolas, G. (1980): Intercellular Junctions in the Adrenal Medulla : A Comparative Freeze Fracture Study, Tissue Cell12:661-672.
21.
Hirvonen, T.J. and Narhi, M. (1986): The Effect of Dentinal Stimulation on Pulp Nerve Function and Pulp Morphology in the Dog, J Dent Res65:1290-1293.
Holland, G.R. (1976): Lanthanum Hydroxide Labelling of Gap Junctions in the Odontoblast Layer, Anat Rec186:121-126.
24.
Holland, G.R. (1980): Non-myclinated Nerve Fibers and Their Terminals in the Subodontoblast Plexus of the Feline Dental Pulp, J Anat130:457-467.
25.
Holland, G.R. (1987): The Effect of Nerve Section on the Incidence and Distribution of Gap Junctions in the Odontoblast Layer of the Cat, Anat Rec218:458-465.
26.
Iguchi, Y.; Yamamura, T.; Ichikawa, T.; Hashimoto, S.; Horiuchi, T.; and Shimono, M. (1984): Intcrccllular Junctions in Odontoblasts of the Rat Incisor Studied with Freeze-Fracture, Arch Oral Biol29:487-497.
27.
Jessen, H. (1967): The Ultrastructure of Odontoblasts in Pcrfusion Fixed, Demineralized Incisors of Adult Rats, Acta Odontol Scand25 :491-523.
28.
Johnson, G.; Olgart, S.; and Brannstrom, M. (1973): Outward Fluid Flow in Dentin Under a Physiologic Pressure Gradient: Experiments in vitro, Oral Surg Oral Med Oral Pathol35:238-248.
29.
Koling, A.; Rask-Andersen, H.; and Bagger-Sjoback, D. (1981): Membrane Junctions on Odontoblasts. A Freeze-Fracture Study, Acta Odontol Scand39:355-360.
30.
Koling, A. and Rask-Andersen, H. (1984a): Membrane Junctions Between Odontoblasts and Associated Cells. A Freeze-Fracture Study of the Human Odontoblastic Cell Layer with Special Reference to its Nerve Supply, Acta Odontol Scand42:13-22.
31.
Koling, A. and Rask-Andersen, H. (1984b): Membrane Structures in the Pulp Dentin Border Zone. A Frceze-fracture Study of Demineralized Human Teeth, Acta Odontol Scand138:355-360.
32.
Marfurt, C.F. and Turner, D.F. (1983): Sensory Nerve Endings in the Rat Orofacial Region Labeled by the Anterograde and Transganglionic Transport of Horseradish Peroxidase: a New Method for Tracing Peripheral Nerve Fibers, Brain Res261:106-117.
33.
Marfurt, C.F.; Turner, D.F.; and Adams, C.E. (1988): Stabilization of Tetramethylbenzidine (TMB) Reaction Product at the Electron Microscopic Level by Ammonium Molybdate, J Neurosci Meths25:215-223.
34.
Matthews, B. and Holland, G.R. (1975): Coupling Between Nerves in Teeth, Brain Res98:354-358.
35.
Mesulam, M.M. (1978): Tetramethyl Benzidine for Horseradish Peroxidase Neurohistochemistry: a Non-carcinogenic Blue Reaction Product with Superior Sensitivity for Visualizing Ncural Afferents and Efferents, J Histochem Cytochem26:106-117.
36.
Nagai, N. and Frank, R.M. (1974): Electron Microscopic Autoradiography of Ca45 During Dentinogencsis, Cell Tissue Res155 :513-523.
37.
Pashley, D.H.; Kepler, E.E.; Williams, E.C.; and Okabe, A. (1983a): The Effects of Acid Etching on the in vivo Permeability of Dentine in the Dog, Arch Oral Biol29:853-858.
38.
Pashley, D.H.; Kepler, E.E.; Williams, E.C.; and Okabe, A. (1983b): Progressive Decrease in Dentine Permeability Following Cavity Preparation, Arch Oral Biol28:853-858.
39.
Pashley, D.H.; Kepler, E.E.; Williams, E.C.; and O'Meara, J.A. (1984): The Effect on Dentine Permeability of Time Following Cavity Preparation in Dogs, Arch Oral Biol29:65-68.
40.
Pashley, D.H.; Livingston, M.J.; and Greenhill, J.D. (1978): Regional Resistance to Fluid Flow in Human Dentine in vitro, Arch Oral Biol23:807-810.
41.
Pashley, D.H.: Nelson, R.; and Williams, E.C. (1981): Dentine Hydraulic Conductance: Changes Produced by Red Blood Cells, J Dent Res60:1779-1802.
42.
Reith, E.J. (1968): Collagen Formation in Developing Molar Teeth of Rats, J Ultrastruct Res21 :383-414.
43.
Sakumoto, T.; Nagai, T.; Kimura, H.; and Maeda, T. (1980): Electron Microscopic Visualization of Tetramethyl-Benzidine Reaction Product on Horseradish Peroxidase Neurohistochcmistry, Cell Mol Biol26:211-216.
44.
Sasaki, T.; Ishida, I.; and Higashi, S. (1982a): Ultrastructure and Cytochemistry of Old Odontoblasts in Rat Incisors, J Electron Microsc31:378-388.
45.
Sasaki, T.; Nakagawa, K.; and Higashi, S. (1982b): Ultrastructurc of Odontoblasts in Kitten Tooth Germs as Rcvcalcd by Freeze-fracture, Arch Oral Biol27:897-904.
46.
Sattelberg, C.F. and Turner, D.F. (1984): Anatomical Evidence for the Existence of Zonula Occludens Between Pulpal Odontoblasts, J Dent Res63:225, Abst. No. 494.
47.
Sattelberg, C. and Turner, D.F. (1985): Permeability of the Pulpal Odontoblast Cell Layer Following Cavity Preparation, J Dent Res64:240, Abst. No. 588.
48.
Stewart, J.M. (1965): The Immediate Response of Odontoblasts to Injury , Odont Tidskr73:417-423.
49.
Takuma, S. and Nagai, N. (1971): Ultrastructure of Rat Odontoblasts in Various Stages of Their Development and Maturation, Arch Oral Biol16:993-1011.
50.
Tanaka, T. (1980): The Origin and Localization of Dental Fluid in Developing Rat Molar Teeth Studied With Lanthanum as a Tracer, Arch Oral Biol25:153-162.
51.
Tice, L.W.; Carter, R.L.; and Cahil, M.C. (1977): Tracer and Freeze-fracture Observations on Developing Tight Junctions in Fetal Rat Thvroid, Tissue Cell9:395-418.
52.
Turner, D.F. (1982): Ultrastructure of the Primate Dental Pulp Organ , Anat Rec202:193A.
