Studies on the High-Sulfur Proteins of Reduced Merino Wool

Abstract

A reinvestigation of a fraction of the high-sulfur proteins SCMKB was made. The proteins were successively fractionated on an electrophoretic, molecular-size, and chromatographic basis. This work resulted in the isolation of a homogeneous protein SCMKB-IIIB2 which has a molecular weight of 11,260, no free amino-terminal group, and contains S-carboxymethyl cysteine as carboxy-terminal residue.

Keywords

Merino Wool Proteins Sulfur Compounds Chemically Modified Protein Fibers Chromatography Filtrations Gels Electrophoresis Fractionation Amino Acids Carboxyl Groups Lysine Cysteine Tryptophan Tyrosine Threonine Chemical Composition

References

1. Braun

Schroeder

W. A.

, Reinvestigation of the Hydrazinolytic Procedure for the Determination of Carbon Terminal Amino Acids, Arch Biochem. Biophys. 118, 241–252 (1967).

2. Flodin

Killander

, Fractionation of Human-Serum Proteins by Gel Filtration, Biochim. Biophys. Acta 63, 403–410 (1962).

3. Gillespie

J. M.

, The Isolation and Properties of Some Soluble Proteins from Wool. Pt. IV: The Isolation of the High-Sulfur Protein SCMKB1, Australian J. Biol. Sci. 15, 572–588 (1962).

4. Gillespie

J. M.

, Isolation and Properties of Some Soluble Proteins from Wool. VII, Australian J. Biol. Sci. 16, 259–280 (1963).

5. Gillespie

J. M.

Harrap

B. S.

, The Isolation and Properties of Some Soluble Proteins from Wool. Pt. VI: The Physiochemical Properties of SCMKB2, Australian J. Biol. Sci. 16, 252–258 (1963).

6. Hill

R. L.

Schmidt

W. R.

, Complete Enzymic Hydrolysis of Proteins, J. Biol. Chem. 237, 389–396 (1962).

7. Joubert

F. J.

Burns

M. A. C.

, Fractionation of the High-Sulfur Proteins of Reduced Merino Wool, J. S. African Chem. Inst. 20, 161–173 (1967).

8. Joubert

F. J.

De Jager

P. J.

Swart

L. S.

, “Symposium on Fibrous Proteins,” Australia, 1967. Ed. Crewther, W. G., Butterworths, Australia, 1968, p. 343.

9. Porath

Lindner

E. B.

Jerstedt

, Migration-Elution of Zones in an Electrophoresis Column, Nature 182, 744–745 (1958).

10.

10. Sanger

Thompson

E. O. P.

, Halogenation of Tyrosine During Acid Hydrolysis, Biochim. Biophys. Acta 71, 468–471 (1963).

11.

11. Sober

H. A.

Gutter

F. J.

Wyckoff

M. M.

Peterson

E. A.

, Chromatography of Proteins. II: Fractionation of Serum Protein on Anion-Exchange Cellulose, J. Am. Chem. Soc. 78, 756–763 (1956).

12.

12. Yphantis

D. A.

, Rapid Determination of Molecular Weights of Peptides and Proteins, Ann. N. Y. Acad. Sci. 88, 586–601 (1960).