Elemental sulphur (as S0 and S8) is abundant in anaerobic sediments and soil, and is highly toxic in the Vibrio fischeri bioluminescence test. This mode of S0 action remains uncertain. The objective of this research was the analysis of the toxic effects of S0 on bioluminescence and respiration in V. fischeri, in joint action with N-ethylmaleimide (NEM) or 2,4-dithio-DL-threitol (DTT), which are –SH group inhibiting and maintaining synthetic agents, respectively. Non-toxic DTT immediately protected cell bioluminescence against S0 inhibition at low (5.5ppb) and high (55ppb) concentrations of S0, whilst restoration of the inhibitory effect of S0 took up to 30 minutes. NEM (62.5ppb) diminished cell bioluminescence by up to 50% after 5 minutes, but after 60 minutes, the inhibition reached 100%. DTT restored the bioluminescence function inhibited in vivo and in vitro by S0 and NEM. Enhancement of cell respiration by up to 20% and 33% was observed at 2.2ppm of S0 and 36.8ppm of 2,4-dinitrophenol (2,4-DNP; an uncoupler of oxidative phosphorylation), respectively; whilst NEM (3.1ppm) caused a reduction of up to 40%. This comparative analysis confirmed that S0 has multiple modes of action — it acts as both an –SH group inhibitor and an uncoupler of oxidative phosphorylation in V. fischeri cells.
ZopfiJ., FerdelmanT.G., & FossingH. (2004). Distribution and fate of sulphur intermediates — sulphite, tetrathionite, thiosulphate, and elemental sulphur — in marine sediments. In Sulphur Biogeo-chemistry — past and present: Boulder Co. (eds. AmendJ.P., EdwardsK.J., & LyonsT.W.). Geological Society of America Special Paper379, 97–116.
2.
PaulE.A., & ClarkF.E. (1996). Sulfur transformations in soil. In Soil Microbiology and Biochemistry (eds. PaulE.A., & ClarkF.E.), 2nd edn, pp. 299–314. San Diego, CA, USA: Academic Press.
3.
MadiganM.T., MartinkoJ.M., & ParkerJ. (1997). Brock Biology of Microorganisms, 8th edn, pp. 175–722. London, UK: Prentice Hall.
4.
AzarovaI.N., GorshkovA.G., GrachevM.A., KorzhovaE.N., & SmagunovaA.N. (2001). Determination of elemental sulfur in bottom sediments using high-performance liquid chromatography. Journal of Analytical Chemistry56, 929–933.
5.
PodgorsekL., & ImhoffJ.F. (1999). Tetrathionate production by sulfur oxidizing bacteria and the role of tetrathionate in the sulphur cycle of Baltic Sea sediments. Aquatic Microbial Ecology17, 255–265.
6.
NelsonD.C., & CastenholzR.W. (1981). Use of reduced sulphur compounds by Beggiatoa sp. Journal of Bacteriology147, 140–154.
7.
Anon. (2000). Water Quality — Determination of the Inhibitory Effect of Water Samples on the Light Emission of Vibrio fischeri (Luminescent Bacteria Test). Part 3: Method Using Freeze-dried Bacteria. LST EN ISO 11348-3:2000. 13 pp., in Lithuanian. Vilnius, Lithuania: Standardisation Department of Lithuania.
8.
JacobsM.W., DelfinoJ.J., & BittonG. (1992). The toxicity of sulphur to Microtox® from acetonitrile extracts of contaminated sediments. Environmental Toxicology and Chemistry11, 1137–1143.
9.
SalizzatoM., PavoniB., Volpi GhirardiniA., & GhettiF. (1998). Sediment toxicity measured using Vibrio fischeri as related to the concentrations of organic (PCBs, PAHs) and inorganic (metals, sulphur) pollutants. Chemosphere36, 2949–2968.
10.
PardosM., BenninghoffC., ThomasR.L., & Khim-HeangS. (1999). Conformation of elemental sulphur toxicity in the Microtox® assay during organic extracts assessment of freshwater sediments. Environmental Toxicology and Chemistry18, 188–193.
11.
SabaliunasD., LazutkaJ.R., & SabaliunieneI. (2000). Acute toxicity and genotoxicity of aquatic hydrophobic pollutants sampled with semipermeable membrane devices. Environmental Pollution109, 251–265.
12.
JeffersC.E., NicholsJ.C., & TuS.C. (2003). Complex formation between Vibrio harveyi luciferase and monomeric NADPH:FMN oxidoreductase. Biochemistry42, 529–534.
13.
LeiB., ChoK.W., & TuS.C. (1994). Mechanism of aldehyde inhibition of Vibrio harveyi luciferase. Identification of two aldehyde sites and relationship between aldehyde and flavin binding. The Journal of Biological Chemistry269, 5612–5618.
14.
LinL.Y.C., SuleaT., SzittnerR., KorC., PurisimaE.O., & MeighenE.A. (2002). Implications of the reactive thiol and the proximal non-proline cis-peptide bond in the structure and function of Vibrio harveyi luciferase. Biochemistry41, 9938–9945.
15.
BaldwinT.O., ChenL.H., ChlumskyL.J., DevineJ.H., & ZieglerM.M. (1989). Site-directed mutagen-esis of bacterial luciferase: analysis of the “essential” thiol. Journal of Bioluminescence and Chemiluminescence4, 40–48.
16.
CetkauskaiteA., PessalaP., & SödergrenA. (2004). Elemental sulphur: Toxicity in vivo and in vitro to bacterial luciferase, in vitro yeast alcohol dehydrogenase, and bovine liver catalase. Environmental Toxicology19, 372–386.
17.
LowJ.C., & TuS.C. (2002). Functional roles of conserved residues in the unstructured loop of Vibrio harveyi bacterial luciferase. Biochemistry41, 1724–1731.
18.
DzejaP., BauzaiteJ., ToleikisA., PraškevičiusA., & JasaitisA. (1993). The interaction between ligands and adenine nucleotide translocase of heart mitochondria. Biologija2, 51–56.
19.
DanilovV.S., & JegorovN.S. (1990). Bacterial Bioluminescence, 152 pp. Moscow, Russia: Moscow State University Publishing House.
20.
BradfordM.M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry72, 248–254.
21.
BerzinskieneJ., & CetkauskaiteA. (1996). Chlorinated acetanilides: biodegradation and toxicity in gram-negative cells. In Environmental Xenobiotics (ed. RichardsonM.), pp. 261–282. London, UK: Taylor & Francis.
22.
Anon. (1994). Determination of the Inhibitory Effect of Water Samples on the Light Emission of Vibrio fischeri (Luminescent Bacteria Test). ISO/TC 147/SC 5/WG 1N 146. 31 pp. Geneva, Switzerland: Internal Standards Organisation.
23.
BourgoisJ.J., SluseF.E., BaguetF., & MallefetJ. (2001). Kinetics of Light Emission and Oxygen Consumption by Bioluminescent Bacteria. Journal of Bioenergetics and Biomembranes33, 353–363.
KohnenM.E.L., & HorsfieldB. (1998). Origin of low-molecular-weight alkylthiophenes in pyrolysates of sulphur-rich kerogens as revealed by micro-scale sealed vessel pyrolysis. Organic Geochemistry29, 1891–1903.
