«In Vivo » Studies on RNA Metabolism of Human Acute Leukemia Cells.

Abstract

Italian

« In vivo » injection of uridine-³H was employed for the direct study of RNA metabolism in acute leukemia cells: blood samples were taken at different times following precursor administration. The degree of RNA labelling in the whole population, in some classes of blast cells with different diameter, and in the single subcellular structures was evaluated by high resolution autoradiographic technique. Great stability was observed in the RNA labelling of the blast population. This finding is discussed in relation to the following points: 1) true metabolic stability, 2) continuous intracellular resynthesis of the catabolites of the labelled macromolecules, 3) reutilization of the extracellular degradation products. From the behaviour of RNA labelling in the different classes and time-stages of the experiment some deductions about the proliferative kinetics of leukemia cells are possible. Finally from the behaviour of the labelling in the different subcellular structures (nucleus, nucleolus, cytoplasm) an early nuclear and a possible nucleolar synthesis of RNA was observed.

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