Osteoblast MC3T3-E1 Culture on a Fast-setting Carbonated Hydroxyapatite Bone-like Material

Abstract

Using ultra-fine grain technology, a fast-setting type of carbonated hydroxyapatite (CHA) material from Ca₃(PO4)₂·3H₂O (ACP), CaCO₃, Ca(H₂PO₄)₂ (MCPA) and Na₃PO₄ was prepared. The ultra-fine particles (3 m) shortens the setting time, to form bone like material with 5.2wt% carbonation. Osteoblastic MC3T3-E1 cells were cultured with CHA material to observe cell adhesion, proliferation and differentiation. There were no obvious differences in adhesion and proliferation rate of MC3T3-E1 cells between the CHA and the control group. MC3T3-E1 cells on the surface of CHA observed by a scanning electron microscope (SEM). The alkaline phosphatase (ALP) activity of the cells on the CHA was higher than the control, and collagen I (COL 1) mRNA expressed on the CHA was also significantly higher than the control. This CHA bone material exhibited biocompatibility and osteo-conductive properties that are sought for bone repair and scaffolding for tissue engineering. *Author to whom correspondence should be addressed.

Keywords

carbonated hydroxyapatite osteoblast biocompatibility bone repair tissue engineering tissue scaffold

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