Besides the quantification of platelet adhesion, the morphology of adherent platelets on a polymer surface seems to be important as far as thrombogenicity is concerned. It is suggested that such events usually take place on a layer of adsorbed proteins on the surface of the blood. In the present study, we observed the morphological variation of adherent calf platelets (in vitro under static conditions) on bare and hydrogel-grafted Angioflex sheets. Electron micrographs indicate the extent of deformation of the Angioflex sheets varying with modified substrates as compared to the bare sheets.
References
1.
Baier, R.E.1977. "The Organisation of Blood Components Near Interfaces," Ann. N.YAcad. Sci., 283:17-36.
2.
Kochwa, S., R.S. Litwak and R.E. Rosenfield .1977. "Blood Elements at Foreign Surfaces: A Biochemical Approach to the Study of the Adsorption of Plasma Proteins," Ann. N.Y. Acad. Sci., 283:37-49.
3.
Sawyer, P.N. , C. Burrowes, J. Ogoniak, A.O. Smith and S.A. Wesolowski.1964. "Ionic Architecture at the Vascular Wall Interface," Trans. Am. Soc. Artif. Intern. Organs, 10:316-319.
4.
Sasaki, T., B.D. Ratner and A.S. Hoffman.1976. "Radiation Induced Co-Graft Polymerization of 2-HEMA and Ethyl Methacrylate onto Silicon Rubber Films," ACS Symposium Series, Hydrogels for Medical and Related Applications , J. D. Andrade, ed., Am. Chem. Soc., 71:283-294.
5.
Hari, P.R. and C.P. Sharma.1991. "Hydrogel Grafted Surfaces: Protein Interaction and Platelet Adhesion," J. Biomat. Appl., 6:170.
6.
Vander Does, L., J. Hoffman and T.E.C. Van Utteren.1973. J Polym. Sci. Polym. Lett., 11:169.
7.
Sharma, C.P. and A.K. Nair.1987. "PE Grafted PEUU Interactions with Blood Proteins and Platelets," 2nd World Congress on Biomaterials, Washington DC, April 27-May 1.
8.
Jansen, B.J.1987. "Chemical Modification of Polyurethanes by Radiation-Induced Grafting," J. Biomat. Appl. , 1:502-532.
