Revisión: E1 polimorfismo del gen de la caseina αs1 caprina y su efecto sobre la producción,la composición y las propiedades tecnológicas de la leche y sobre la fabricación y la maduración del queso
Available accessResearch articleFirst published online August, 1998
Revisión: E1 polimorfismo del gen de la caseina αs1 caprina y su efecto sobre la producción,la composición y las propiedades tecnológicas de la leche y sobre la fabricación y la maduración del queso
The caprine α s1-casein gene displays a high degree of polymorphism, with eight different protein variants described up to date. The rate of synthesis of the mature protein is closely linked to the caprine αs1-casein genotype. There are three variants which are associated to high αs1-casein content in milk (A, B, and C), one to an intermediate content (E), three to a low content (D, F, and G) and one to a null content (O). During the last few years studies of goat milk protein polymorphism have gained renewed interest, as the ocurrence of certain milk protein genetic variants is correlated with the composititon of milk and also with some milk processing parameters. For instance, analyses of the physicochemical properties of milk from goats homozy gous for the three main α s1-casein alleles (A, E, F) confirm the effects of the genotypes on the percentage of casein and fat, and shows significant effects on the diameter of the micelles and their calcium content. These characteristics seem to be associated with enhanced clotting of goat milk, cheese curd firmness, curd syneresis and overall cheese yield. The caprine αs1-casein gene constitutes a very attractive model for studying the regulation of the expression of the corresponding protein, and might also become a very powerful tool in improving the technolog ical properties of goat milk.
Alexander L.J. y Beattie C.W. (1992). The sequence of the porcine αs1-casein cDNA: evidence for protein variants generated by altered RNA splicing. AnimalGenetics23: 283-288.
2.
Ambrosoli R., Di Stasio I.y Mazzocco P. (1988). Content of αs1-casein and coagulation properties in goat milk. Journal of Dairy Science71: 24-28.
3.
Amills M. (1996). Diseño y aplicación de técnicas moleculares para el análisis del efecto del polimorfismo de la caseína αs1 caprina sobre caracteres productivos. Tesis doctoral Universitat Autònoma de Barcelona. Barcelona, España.
4.
Amills M., Francino O., Jansà M. y Sànchez A. (1997). Isolation of genomic DNA from milk samples by using Chelex resin. Journal of Dairy Research64: 231-238.
5.
Angulo C., Amills M., Ares J.L., Jiménez I., Jordana J., Sànchez A. y Serradilla, J.M. (1996). Genetics of caseins and its relation with yield, composition and cheese making of milk in Spanish breeds of goats. En: Proceedings of the 47th Annual Meeting of the European Association of Animal Production Lillehammer, Noruega: p. 209.
6.
Barbieri M.E., Manfredi M., Elsen J.M., Ricordeau G., Bouillon J., Grosclaude F., Mahé M.F. y Bibé B. (1995). Influence du locus de la caséine αs1 sur les performances laitières et les paramètres génétiques des chèvres de race Alpine. Génétique Sélection etEvolution27: 437-450.
7.
Boulanger A., Grosclaude F. y Mahé M.F. (1984). Polymorphisme des caséines αs1 et αs2 de la chèvre (Capra hircus). Génétique Sélection etEvolution16: 157-176.
8.
Bouniol C. (1993). Sequence of the goat αs2-casein encoding cDNA . Gene125: 235-236.
9.
Bouniol C., Brignon G., Mahé M.F. y Printz C. (1994). Biochemical and genetic analysis of variant C of caprine αs2-casein (Capra hircus). AnimalGenetics25: 173-177.
10.
Brignon G., Mahé M.F., Grosclaude F. y Ribadeau-Dumas B. (1989). Sequence of caprine αs1-casein and characterization of those of its genetic variants which are synthesized at a high level, αs1-Cn A, B and C. Protein Sequence and Data Analysis2: 181-188.
11.
Brignon G., Mahé M.F., Ribadeau-Dumas B., Mercier J.C. y Grosclaude F. (1990). Two of three genetic variants of goat αs1-casein which are synthesized at a reduced level have an internal deletion probably due to altered RNA splicing. EuropeanJournal of Biochemistry193: 237-41.
12.
Caroli A., Maccarini G., Bolla P. y Pagnacco, G. (1996). Effect of αs1-casein polymorphism on milk quality traits in Saanen and Alpine goats bred in Italy. En: Proceedings of the XXV International Conference on Animal Genetics. Tours, Francia: pp. 173-174.
13.
Chianese L., D'Auria R., Ferranti P., Garro G., Mauriello R., Rubino R.,. y Addeo F. (1996). Occurrence of novel αs1-casein variants in Italian goat breeds. En: Production and Utilization of Ewes and Goats milk . Brussels: IDF. pp. 141-147.
14.
Ciafarone N. y Addeo F. (1984). Composizione della caseina e propietà del latte di capra. Il Vergaro11: 17-24.
15.
Coll, A. (1995). Caracterització del gen de la κ-caseïna per a la seva utilització com a transgen. Tesis Doctoral, Universitat Autònoma de Barcelona. Barcelona, España.
16.
Coll A., Folch J.M. y Sànchez A. (1995). Structural features of the 5' flanking region of goat κ-casein. Journal of Dairy Science78: 973-977.
17.
Creamer L.K.y Olson N.F. (1982). Rheological evaluation of maturing Cheddar cheese . Journal of Food Science47: 631-636,646.
18.
Delacroix-Buchet A., Degas C., Lamberet G. y Vassal L. (1996). Influence des variants AA et FF de la caséine αs1 caprine sur le rendement fromager et les caractéristiques sensorielles des fromages. Lait76: 217-241.
19.
Díaz-Carrillo E. (1993). Análisis de los contenidos y producción total de las fracciones caseínicas en la leche de tres razas de cabras españolas. Factores de variación y relación con otros componentes lácteos. Tesis Doctoral, Universidad de Córdoba. Córdoba , España.
20.
Díaz-Carrillo E., Múñoz Serrano A., Alonso Moraga A. y Serradilla Manrique J.M. (1993). Near infrared calibrations for goat's milk components: protein, total casein, αs, β, and κ caseins, fat and lactose. Journal of Near Infrared Spectroscopy1: 141-146.
21.
Díaz-Carrillo E., Angulo-Heras C., Jordana J., Amills M., Sànchez A. y Serradilla J.M. (1994). Casein variation in one Spanish breed of goats and their relationships with production traits. En: 45th Annual Meeting of the European Association for Animal Production . Edinburgh, UK. p. 296.
22.
DiLuccia A., Mauriello R., Chianese L., Moio L. y Addeo F. (1990). κ-casein polymorphism in caprine milk. Scienze e Tecnologia Lattearia e Casearia41: 305-314.
23.
Dupuy V. (1991). Acquis scientifiques de la Station de Testage Caprin de Moissac Vallée-Française et effets du polymorphisme génétique de la caséine αs1 de chèvre sur les perfomances laitières. Diplome d'Agronomie Approfondie, Ecole Nationale Superieure Agronomique de Montpellier.
24.
Eigel W., Butler J.E., Ernstrom C.A., Farrell H.M., Harwalkar V.R., Jenness R. y Whitney R. McL. (1984). Nomenclature of proteins of cow's milk: fifth revision. Journal of Dairy Science67: 1599-1631.
25.
Ernst C.A. y Dentine M.R. (1992). Genotyping of dairy cattle using direct amplification from somatic cells in small volumes of milk. Animal Genetics23 (Suppl. 1): 56.
26.
Ferretti L., Leone P. y Sgaramella V. (1990). Long range restriction anlaysis of the bovine casein genes. Nucleic Acids Research18: 6829-6833.
27.
Fox P.F. (1989). The milk protein system. En: Fox P.F. (ed.), Developments in Dairy Chemistry. London: Elsevier Applied Science Publishers Ltd. pp. 1-54.
28.
Grosclaude F., Joudrier P. y Mahé M.F. (1978). Polymorphisme de la caséine αs2 bovine: étroite liaison du locus αs2-Cn avec les loci αs1-Cn, β-Cn et κ-Cn; mise en évidence d'une délétion dans le variant αs2-Cn D. Annals de Génétique et de Sélection Animal10: 313-327.
29.
Grosclaude F., Mahé M.F., Brignon G., Di Stasio L. y Jeunet R. (1987). A Mendelian polymorphism underlying quantitative variations of goat αs1-casein. Génétique Sélection et Evolution19: 399-412.
30.
Grosclaude F., Ricordeau G., Martin P., Remeuf F., Vassal L. y Bouillon J. (1994). Du gène au fromage: le polymorphisme de la caséine αs1 caprine, ses effets, son évolution. INRA Production Animal7(1): 3-19.
31.
Ha J.K. y Lindsay R.C. (1991). Contributions of cow, sheep, goat milks to characterizing branched-chain fatty acid and phenolic flavors in varietal cheeses. Journal of Dairy Science74: 3267-3274.
32.
Hayes H., Petit E., Bouniol C. y Popescu P. (1993). Localization of the αs2-casein gene (CASAS2) to the homeologous cattle, sheep and goat chromosomes 4 by in situ hybridization . Cytogenetics and Cell Genetics64: 281-285.
33.
Heil F. y Dumont J.P. (1993). Caractéristiques organoleptiques de fromages de chèvre fabriqués à partir de laits contenant des variants génétiques différents de la caséine αs1. Lait73: 559-565.
34.
Hill R.D., Lahav E. y Givol D. (1974). A rennin-sensitive bond in αs1-B casein. Journal of Dairy Research41: 147-153.
35.
Jairam B.T. y Nair P.G. (1983). Genetic polymorphisms of milk proteins and economic characters in dairy animals. Indian Journal of Animal Sciences53: 1-8
36.
Jansà M. (1995). Anàlisi comparativa estructural i funcional del polimorfisme del gen de la caseína αs1 cabrum. Tesis Doctoral, Universitat Autònoma de Barcelona. Bellaterra, España.
37.
Jansà M., Leroux C., Sànchez A. y Martin P. (1994). Occurrence of a LINE sequence in the 3' UTR of the goat αs1-casein E-encoding allele associated with reduced protein synthesis level. Gene147: 179-187.
38.
Jaubert A. y Martin P. (1992). Reverse-phase HPLC analysis of goat caseins. Identification of the αs1 and αs2 genetic variants. Lait72: 235-247.
39.
Johnsen L.B., Rasmussen L.K., Petersen T.E. y Berglund L. (1995). Characterization of three types of human αs1casein mRNA transcripts. Biochemistry Journal309: 237-242.
40.
Jordana J., Amills M., Diaz E., Angulo C., Serradilla J.M. y Sànchez A. (1996). Gene frequencies of caprine αs1-casein polymorphism in Spanish goat breeds. Small Ruminant Research20: 215-221.
41.
Karl V., Guster J., Dietrich A., Maas B. y Mosandl A. (1994). Stereisomeric flavour compounds. LCVIII. 2-alkyl-branched, 3-alkyl-branched, and 4-alkyl-branched acids. 2. Chirospecific analysis and sensory evaluation. Chirality6: 427-434.
42.
Koczan D., Hobom G. y Seyfert H.M. (1991). Genomic organization of the αs1-casein gene . Nucleic Acids Research19: 5591-5596.
43.
Lamberet G., Degas C., Delacroix-Buchet A. y Vassal L. (1996). Influence de caractères liés aux allèles A et F de la caséine αs1 caprine sur la flaveur chèvre: fabrications fromagères avec échange de protéines et de matières grasses. Lait76: 349-361.
44.
Law A.J.R. y Tziboula A. (1992). Quantitative fractionation of caprine casein by cation-exchange FPLC. Milchwissenschaft47: 558-562.
45.
Le Quéré J.L., Demaizières D., Negrello C., Lesschaeve I., Issanchou S. y Salles C. (1996). Goat cheese flavour. Identification of character flavour impact compounds. En: (eds.), IDF Symposium, Besançon , Francia: IDF. pp. 205-208.
46.
Leroux C., Martin P., Mahé M.F., Levéziel H. y Mercier J.C. (1990). Restriction fragment length polymorphism identification of goat αs1-casein alleles. A potential tool in selection of individuals carrying alleles associated with a high level protein synthesis. Animal Genetics21: 341-351.
47.
Leroux C., Mazure N. y Martin P. (1992). Mutations away from splice site recognition sequences might cis-modulate alternative splicing of goat αs1-casein transcripts . Journal of Biological Chemistry267: 6147-6157.
48.
Leroux C. y Martin P. (1996). The caprine αs1- and β-casein genes are 12-kb apart and convergently transcribed. Animal Genetics27 (suppl. 2): 93.
49.
Lin C.Y., McAllister C.J., Ng-Kwai-Hang K.F. y Hayes J.F. (1986). Effects of milk protein loci on first lactation production in dairy cattle. Journal of Dairy Science69: 704-712.
50.
Lipkin E., Shalom A., Khatib H., Soller M. y Friedmann A. (1992). Milk as a source of deoxyribonucleic acid and as a substrate for the polymerase chain reaction. Journal of Dairy Science76: 2025-2032.
51.
Lodes A., Krause I., Buchberger J., Aumann J. y Klostermeyer H. (1996). The influence of genetic variants of milk proteins on the compositional and technological properties of milk. 1. Casein micelle size and the content of non-glycosylated κ-casein. Milchwissenschaft51(1): 368-373.
52.
Mahaut M. y Korolczuk J. (1993). Effect of genetic variants of casein on the recovery of milk proteins in fresh and soft cheeses obtained by ultra- or microfiltration from goat's milk. En: Cheese Yield and Factors Affecting its Control. IDF Seminar, Cork, UK: IDF. pp. 175-178.
53.
Mahé M.F. y Grosclaude F. (1989). αs1-Cn D, another allele associated with a decreased synthesis rate at the caprine αs1-casein locus. Génétic Sélection et Evolution21: 127-129.
54.
Mahé M.F. y Grosclaude F. (1993). Polymorphism of β-casein in Creole goat of Guadeloupe: evidence for a null allele. Génétic Sélection et Evolution25: 403-408.
55.
Mahé M.F., Manfredi E., Ricordeau G., Piacère A. y Grosclaude F. (1994). Effets du polymorphisme de la caséine αs1 caprine sur les perfomances laitières: analyse intradescendance de boucs de race Alpine. Génétic Sélection et Evolution26: 151-157.
56.
Manfredi E., Barbieri M.E., Bouillon J., Piacère A., Mahé M.F., Grosclaude F. y Ricordeau G. (1993). Effets des variants de la caséine αs1 sur les perfomances laitières de chèvres. Lait73: 567-572.
57.
Manfredi E., Ricordeau G., Barbieri M.E., Amigues Y. y Bibé B. (1995). Génotype caséine αs1 et sélection des boucs sur descendance dans les races Alpine et Saanen. Génétic Sélection et Evolution27: 451-458.
58.
Martin P. y Leroux C. (1994). Characterization of a further αs1-casein variant generated by exon skipping. Animal Genetics25 (suppl. 2): 34.
59.
Marziali A.S. y Ng-Kwai-Hang K. F. (1986). Effect of milk composition and genetic polymorphism on cheese composition. Journal of Dairy Science69: 2533-2542.
60.
McLean D.M., Graham E.R.B., Ponzoni R.W. y McKenzie H.A. (1984). Effects of milk protein genetic variants on milk yield and composition. Journal of Dairy Research54: 219-235.
61.
Medrano J.F. y Aguilar-Cordova E. (1990). Genotyping of bovine κ-casein loci following DNA sequence amplification. Bio/Technology8: 144-146.
62.
Mercier J.C. y Grosclaude F. (1993). Génétique moleculaire des protéines et des leurs gènes. En: Martinet J. y Houdebine L.M. (eds.), Biologie de la Lactation . Paris: INSERM/INRA Editions . pp. 319-347.
63.
Mohr U., Koczan D., Linder D., Hobom G. y Erdhardt G. (1994). A single point mutation results in A allele specific exon skipping in the bovine αs1-casein mRNA. Gene143: 187-192.
64.
Mora—Gutiérrez A., Kumosinski T.F. y Farrell H.M.J. (1991). Quantification of αs1-casein goat milk from French-Alpine and Anglo-Nubian breeds using reversed-phase high performance liquid chromatography. Journal of Dairy Science74: 3303-3307.
65.
Morini D., Losi G., Castagnetti G.B., Beneveilli M., Resmini P. y Volonterio G. (1976). The influence of genetic variants of kappa-casein on the size of casein micelles. Scienze e Tecnología Lattearia e Casearia30: 243-260.
66.
Morini D., Losi G., Castagnetti B. y Mariani P. (1979). Prove di caseificazione con latte caratterizzato dalle varianti A e B del κ-caseina: rilievi sul formaggio staglionato . Scienze e Tecnología Lattearia e Casearia30: 243-262.
67.
Natour S. y Seyfert, H.M. (1994). Quantitative control of casein synthesis: delineation of an αs1-RNA sequence motif conferring hormonal mRNA stabilization in cattle. Animal Genetics25 (suppl. 2): 39-40.
68.
Ng-Kwai-Hang K.F., Hayes J.F., Moxley J.E. y Monardes H.G. (1986). Relationships between milk protein polymorphisms and major milk constituents in Holstein-Friesian cows. Journal of Dairy Science69: 22-26.
69.
Ng-Kwai-Hang K.F. y Grosclaude F. (1992). Genetic polymorphism of milk proteins. En: Fox P.F. (ed.), Advanced Dairy Chemistry. London: Elsevier Science Publishers. pp. 405-455.
70.
Ono T. y Creamer L.K. (1986). Structure of goat casein micelles. Nezv Zealand Journal of Dairy Science and Technology21: 57-64.
71.
Pépin L. (1995). Recherche de polymorphisme génétique chez les caprins. Applications à l'étude de la diversité des populations, au contrôle de filiation et à la résistance génétique à la cowdriose. Tesis Doctoral, Université de Paris XIOrsay.
72.
Persuy M.A., Printz C., Medrano J.F. y Mercier J.C. (1996). One mutation might be responsible for the absence of β-casein in two breeds of goats. Animal Genetics27 (suppl. 2): 96.
73.
Pierre A., Le Quéré J.-L. (1995). Cheeses from goat milks with or without αs1-casein . Seminar on Production and Utilization of Ewes and Goats' milk. Limin-Hersonissos, Creta: International Dairy Federation.
74.
Pierre A., Michel F. y Le Graet Y. (1995). Variation in size of goat milk casein micelles related to casein genotype. Lait75: 489-502.
75.
Pinder S.J., Perry B.N., Skidmore C.J. y Savva D. (1991). Analysis of the polymorphism in the bovine casein genes by the use of polymerase chain reaction. Animal Genetics22: 11-22.
76.
Pirisi A., Colin O., Laurent F., Scher J. y Parmentier M. (1994). Comparison of milk composition, cheesemaking properties and textural characteristics of the cheese from two groups of goats with a high or low rate of αs1-casein synthesis. International Dairy Journal4: 329-45.
77.
Ramunno L., Rando S., Di Gregorio P., Massina M. (1994). Indigine di popolazione sui geni e effetto maggiore sul contenuto di caseine αs1 e β nel latte di capra. Zoot Nutr Anim20: 107-111.
78.
Rando A., Pappalardo M., Capuano M., Di Gregorio P. y Ramunno L. (1996). Two mutations might be responsible for the absence of β-casein in goat milk. Animal Genetics27 (suppl. 2): 31.
79.
Remeuf F. (1993). Influence du polymorphisme génétique de la caséin αs1 caprine sur les caractéristiques physico-chimiques et technologiques du lait . Lait73: 549-557.
80.
Remeuf F., Lenoir J. y Duby C. (1989). Etude des relations entre les caractéristiques physico-chimiques des laits de chèvre et leur aptitude à la coagulation par la présure. Lait69: 499-518.
81.
Remeuf F., Cossin V., Dervin C., Lenoir J. y Tomassone R. (1991). Relations entre les caractères physico-chimiques des laits et leur aptitude fromagère. Lait71: 397-421.
82.
Remeuf F., Verdalet-Guzmlan I. y Lenoir J. (1995). Technological adjustment of goat milk containing low synthesis-rate αs1-caseins variants. International Dairy Journal5: 381-392.
83.
Ricordeau G. (1981). Genetics: breeding plans. En: C. Gall (ed.), Goat Production. London: Academic Press. pp. 111-169.
84.
Ricordeau G., Piacere A., Manfredi E. y Amigues Y. (1995). Fréquences alléliques de la caséine αs1 chez les boucs d'insémination de race Alpine et Saanen de 1975 à 194. INRA Production Animal8(4): 259-264.
85.
Roberts B., Di Tullio P., Vitale J., Hehir K. y Gordon K. (1992). Cloning of the goat β-casein gene and expression in transgenic mice. Gene121: 255-262.
86.
Saiki R.K., Scharf S., Faloona F., Mullis K.B., Horn G.T., Erlich H.A. y RNAheim N. (1985). Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia . Science230: 1350-1354.
87.
Sauvant D., Bas P., Ternois F. y Ricordeau G. (1993). Preliminary results on the influence of the αs1-casein variants on the goat milk fatty acids composition. En: Journées Scientifiques et Techniques du Lait de Chèvre. Surgères, France.
88.
Swaisgood H.E. (1993). Genetic perspectives on milk proteins: comparative studies and nomenclature. Review and update of casein chemistry. Journal of Dairy Science76: 3054-3061.
89.
Thompson M.P., Tarassuk N.P., Jennsess R., Lillevik H.A., Ashworth V.S. y Rose D. (1965). Nomenclature of the proteins of cow's milk. Second revision. Journal of Dairy Science48: 159-163.
90.
Threadgill D.W. y Womack J.E. (1990). Genomic analysis of the major bovine casein genes . Nucleic Acids Rescarch18: 6935-6942.
91.
Trujillo A.J. (1996). Proccsos de proteolisis primaria que intervienen en In maduración del queso de cabra. Tesis Doctoral, Universitat Autònoma de Barcelona. Barcelona, España.
92.
Trujillo A.J., Delacroix-Buchet A., Miranda G. y Le Bars D. (1998). Proteolytic specificity of chymosin on caprine αs1caseins A and F. Journal of Dairy Research65 (en prensa).
93.
Tziboula A. (1995). Influence of genetic polymorphism of caprine caseins on the physicochemical properties of caprine milk. Seminar on Production and Utilization of Ewes and Goats' milk. Limin-HersonissosCrete. International Dairy Federation.
94.
Van Eenenaam A.L. y Medrano J.F. (1991). Differences in allelic protein expression in the milk of heterozygous κ-casein cows. Journal of Dairy Science74: 1491.
95.
Vassal L., Delacroix-Buchet A. y Bouillon J. (1994). Influence des variants AA EE et FF de la caséine αs1 caprine sur le rendement fromager et les caractéristiques sensorielles de fromages traditionnels: premières observations. Lait74: 89-103.
96.
Visser S., Slangen Ch.J. y Rollema H.S. (1991). Phenotyping of bovine milk proteins by reversed-phase high-performance liquid chromatography. Journal of Chromatography548: 361-370.
97.
Weiner A.M., Deininger P.L. y Efstratiadis A. (1986). Nonviral retroposons: genes, pseudogenes and transposable elements generated by the reverse flow of genetic information. Annual Reviews in Biochemistry55: 631-61.
98.
Zadow J.G., Hardham J.F., Kocak H.R. y Mayes J.J. (1983). The stability of goat's milk to UHT processing . Australian Journal of Dairy Technology38: 20-23.
