Data on cytokine mRNA expression in CSF and peripheral blood mononuclear cells from MS patients as detected by PCR

Abstract

Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify the mRNA coding for different cytokines in peripheral blood mononuclear cells (PBMC) and cerebrospinal fluid (CSF) cells from 18 multiple sclerosis (MS) patients as compared with 21 other neurological patients. mRNA levels were quantitated by radioactive hybridization of the PCR products. Expression of tumor necrosis factor (TNF)-a, interferon (IFN)-g, and interleukin (IL)-10 mRNA was elevated in CSF cells of MS patients. In many MS patients, both proinflammatory and immunoregulatory cytokine messages were detected in the CSF compartment. Such immune reactivity of CSF cells, as opposed to PBMC, was not associated with higher clinical activity of the disease. Expression of the B7.1 accessory molecule mRNA was similarly investigated. In the CSF, it was detected only in some clinically active MS cases and in other inflammatory diseases.

Keywords

multiple sclerosis cytokine polymerase chain reaction cerebrospinal fluid

References

Hartung HP. (1993) Immune-mediated demyelination . Ann Neurol 33: 563-567 .

Thompson CB. (1995) Distinct roles for costimulatory ligands B7-1 and B7-2 in T helper cell differentiation? Cell 81: 979-982 .

Kuchroo VK et al. (1995) B7-1 and B7-2 costimulatory molecules activate differentially the Th1/Th2 developmental pathways: application to autoimmune disease therapy . Cell 80: 707-718 .

Poser CM et al. (1983) New diagnostic criteria for multiple sclerosis: guidelines for research protocols . Ann Neurol 13: 227-231 .

Link J et al. (1994) Increased transforming growth factor-b, interleukin-4, and interferon-g in multiple sclerosis . Ann Neurol 36: 379-386 .

Navikas V et al. (1995) Increased mRNA expression of IL- 10 in mononuclear cells in multiple sclerosis and optic neuritis . Scand J Immunol 41: 171-178 .

Navikas V et al. (1996) Augmented expression of tumour necrosis factor-alpha and lymphotoxin in mononuclear cells in multiple sclerosis and optic neuritis . Brain 119: 213-223 .

Rieckmann P et al. (1994) Cytokine mRNA levels in mononuclear blood cells from patients with multiple sclerosis . Neurology 44: 1523-1526 .

Rieckmann P et al. (1995) Semi-quantitative analysis of cytokine gene expression in blood and cerebrospinal fluid cells by reverse transcriptase polymerase chain reaction . Res Exp Med 195: 17-29 .

10.

Rieckmann P et al. (1995) Tumor necrosis factor-a messenger RNA expression in patients with relapsingremitting multiple sclerosis is associated with disease activity . Ann Neurol 37: 82-88 .

11.

Beck J et al. (1995) Increased production of interferon gamma and tumor necrosis factor precedes clinical manifestation in multiple sclerosis: do cytokine trigger off exacerbations? Acta Neurol Scand 78: 318-323 .

12.

Sharief MK , Hentges R. (1991) Association between tumor necrosis factor-a and disease progression in patients with multiple sclerosis . New Engl J Med 325: 467-472 .

13.

Chofflon M et al. (1992) Tumor necrosis factor alpha production as a possible predictor of relapse in patients with multiple sclerosis . Eur Cytokine Netw 3: 523-531 .

14.

Bell JI , Lathorp GM. (1996) Multiple loci for multiple sclerosis . Nature Genet 13: 377-378 .