Bioactive calcium phosphate–based glasses and ceramics and their biomedical applications: A review

Abstract

An overview of the formation of calcium phosphate under in vitro environment on the surface of a range of bioactive materials (e.g. from silicate, borate, and phosphate glasses, glass-ceramics, bioceramics to metals) based on recent literature is presented in this review. The mechanism of bone-like calcium phosphate (i.e. hydroxyapatite) formation and the test protocols that are either already in use or currently being investigated for the evaluation of the bioactivity of biomaterials are discussed. This review also highlights the effect of chemical composition and surface charge of materials, types of medium (e.g. simulated body fluid, phosphate-buffered saline and cell culture medium) and test parameters on their bioactivity performance. Finally, a brief summary of the biomedical applications of these newly formed calcium phosphate (either in the form of amorphous or apatite) is presented.

Keywords

In vitro bioactivity glass ceramic calcium phosphate

Introduction

There is a high need for smart off-the-shelf materials that are capable of confirming to the shape of tissues being replaced, modulating cellular function and promoting tissue regeneration. These materials could potentially provide morphological^1–3 or biochemical cues^4,5 that guide cellular interaction which is essential for tissue regeneration. Examples of these smart materials include bioactive glasses and ceramics that undergo specific surface reactions when incubated in simulated body fluid (SBF)⁶ or implanted in animal or human body⁷ leading to the formation of hydroxyapatite (HA) layer that forms a strong bond with the host tissues. They are osteoinductive and osteoconductive; therefore, they attracted much interest in bone tissue engineering.⁸ They can also be used for soft tissue regeneration⁹ and drug delivery applications.¹⁰

Bioactive glasses

These glasses are amorphous and can be prepared either by melt–quench or sol–gel process.¹¹ The sol–gel technology allows for incorporation of biomolecules, proteins or drugs that can be delivered in situ.^12,13 Generally, these glasses can be tailored to release metallic ions that have antimicrobial^14,15 or angiogenic potentials.^16,17 They can also be prepared as solid or nano-/micro-/macro-porous scaffolds¹⁸ with highly ordered, controlled pore size and pores interconnectivity.¹⁹ The porous configuration is necessary for cell migration, angiogenesis and tissue infiltration; this further enhances the bond to the host tissues.²⁰ Functionalisation of the scaffold with amino or carboxylic groups²¹ or loading anti-osteoporotic drugs (e.g. ipriflavone)²² could also be achieved to further improve bone regeneration. Microspheres can also be prepared²³ using different techniques (flame spheroidisation,²⁴ laser²⁵ and thermally induced phase separation and/or oil-in water processing²⁶) for various applications such as radiotherapy,²⁷ drug delivery²² and tissue engineering.²⁶

Under this category, silicate, phosphate and borate glasses will be discussed.

Silicate-based glasses

Silicate glasses are mainly based on SiO₂ (the glass network former); other modifying oxides are also included as Na₂O, CaO and P₂O₅ to adjust the properties of the produced glass. These oxides are usually included in specific molar ratios to produce a biologically active glass. To improve the bioactivity of these glasses, three important compositional features, including (1) SiO₂ content <60 mol%, (2) high Na₂O and CaO content and (3) high CaO/P₂O₅ ratio,²⁰ must be fulfilled. During the glass preparation, a spontaneous crystallisation is undesirable as it reduces the rate of HA formation on its surface.²⁸ The rate of HA layer formation is highly dependent on the degradation of glass. Accordingly, the presence of Na₂O and other alkali or alkaline earth metals increases the rate of HA layer formation. The presence of high silica content or multivalent ions, for example, boron and aluminium, reduces the rate of glass breakdown and hence the apatite layer formation rate.²⁹ Due to their texture (pore size/volume and high surface area), sol–gel-produced glasses showed higher bioactivity than the melt-quenched counterparts.¹¹ Furthermore, using the sol–gel technique, the silica content can be increased from 60 mol% in melt-quenched to 85 mol% in sol–gel without reducing the bioactivity.³⁰

A family of silicate-based glasses have been used for dental and orthopaedic applications²⁹ since 1960s under the commercial name of 45S5 Bioglass™. This formulation has been used as a benchmark for measuring the properties of new silicate glass compositions. When doped with boron, 45S5 Bioglass showed angiogenic potential caused by their ionic dissolution products.^17,31 Boron³² and silver¹⁴ also induced antibacterial action to bioactive silicate glasses.

Silicate-based glasses, however, require high melting temperature during their manufacture, and the addition of various metal oxides to reduce the melting temperature could adversely affect the glass bioactivity. The compositional range and form of this glass is also limited. The degradation of this glass often takes 1–2 years to totally degrade,¹¹ and the long-term effect of silica is still questionable.³³ Fabrication of fibrous scaffold from this glass is also difficult. The search for new bioactive materials that could overcome the limitations of silicate-based glasses has led to the emergence of phosphate and borate-based glasses as alternatives. These glasses can be easily formed without significant crystallisation during their preparation.

Phosphate-based glasses

This class of glasses is mainly based on P₂O₅ (the glass network former), Na₂O and CaO. Other modifying oxides, for example, CuO,¹⁵ ZnO,³⁴ Ag₂O,³⁵ Fe₂O₃,³⁶ TiO₂³⁷ and SrO,³⁸ can also be included to induce a specific property, function or different biological response.^39–41 Unlike silicate glasses, the phosphate tetrahedral has one terminal oxygen; this reduces the network connectivity and hence the rigidity but increases the compositional range of the produced glasses.⁴² Unlike vitreous silica, P₂O₅ is chemically unstable; addition of metal oxides improves its stability.⁴³ The degradation of these glasses varies from hours to years according to the composition and intended applications.

These glasses can be prepared in different forms including discs,^44–46 microtubes,⁴⁷ microspheres^24,48 and fibres.^36,41,49 Fibres can be used as cell transportation and expansion device,⁴⁸ nerve conduit⁵⁰ or as a scaffold for muscle regeneration.⁹ Fibres with antibacterial properties (e.g. copper-containing) can be produced as wound dressing meshes for the treatment of leg ulcers and severe burns.¹⁵ The phosphate glass fibres have an intriguing ability to form microtubes; therefore, they can be incorporated within various polymers to help in diffusion of nutrients and ingrowth of vascularisation when used as scaffolds for soft and hard tissue regeneration.⁵¹

Phosphate glass microspheres were also prepared²³ for radiotherapy²⁷ applications. The morphology of microspheres provides a stable surface for cells to attach and proliferate²⁴ and prevent tissue damage and haemorrhage when used for radiotherapy.²⁷ The spherical morphology would provide large interstitial spaces that can be consistent and quantifiable for cell growth and proliferation than randomly shaped particles when packed into perfusion bioreactors.²⁴

Borate-based glasses

Using borate $({BO}_{3}^{3 -})$ in the glass network provides faster degrading glasses with rapid and complete conversion into HA than silicate-based glasses.⁸ Controlling the boron content tailors the degradation rate of these glasses.⁵² Boron has also beneficial action on bone remodelling and repair.⁵³ Furthermore, the presence of boron may reduce the possibility of bacterial infection through its antimicrobial action.³² An example of borate-based glasses is D-AlK-B (double alkali borate) glass, based on Na₂O–K₂O–MgO–CaO–SiO₂–P₂O₅–B₂O₃ system.⁵⁴

These glasses supported in vitro cell proliferation⁵⁵ and in vivo tissue formation;⁵⁶ they could also be used as drug delivery vehicles.⁵⁷ However, the main concern with these glasses is their potential toxicity.⁸ The degradation products of certain concentration produced an inhibitory effect on the growth of goat bone marrow stromal cells. Adjusting the pH of the glass extract and reducing the concentration of boron to be less than 2.96 mM were observed to stimulate the cell proliferation. Adjusting the boron content to get a reasonable cellular response may jeopardise the bioactivity of these glasses.⁵⁴ The toxicity could also be reduced by dynamic culture conditions.⁵⁸

Ceramics

Another class of bioactive materials include calcium phosphate (CaP)-based ceramics (i.e. crystalline materials), for example, HA, β-tricalcium phosphate (β-TCP) and biphasic CaP (a mixture of HA and β-TCP).

HA can be produced as solid or porous materials. The porous configuration with pores <10 µm in diameter is essential for circulation of body fluids and those >100 µm are essential for colonisation of target cells.²⁰ HA is normally sintered above 1000°C in a granular or block form; after sintering, it cannot be reshaped (if they are present in block form) to fit the defect and it is non-degradable. β-TCP, however, is degradable. The degradation of biphasic CaP is highly dependent on the ratio of its components; the higher the β-TCP content, the faster the degradation. Generally, the degradation of CaP ceramics varies according to their type, porosity, surface area (granular vs blocks) and degree of crystallinity (high crystallinity means low degradation).⁵⁹

Injectable CaP ceramics are also available. They can be easily delivered through a minimally invasive method into the defect as aqueous-based paste. They then set, fill the defect and support tissue regeneration over time.⁶⁰ This allows for their use as drug delivery vehicle⁶¹ or treating a defect in challenging areas, for example, craniofacial complex⁵⁹ or vertebroplasty.⁶² Examples of CaP ceramics that are commercially available include Norian^® (Synthes Craniomaxillofacial, USA), BoneSource^® (Stryker Leibinger, Germany) and Mimix^® (Walter Lorenz Surgical, USA).⁵⁹

Glass-ceramic materials

Glass-ceramics are partially crystallised glasses that are produced by heating the parent glass above its crystallisation temperature.⁶³ Unlike spontaneous surface crystallisation, which is undesirable during glass manufacturing, the crystallisation process is controlled. As a result, the produced glass-ceramics contain one or more crystalline phases embedded in a residual glassy phase.⁶⁴ The bioactivity of glass-ceramics is highly dependent on proportion and type of crystals formed during crystallisation process.⁶⁵ Controlled crystallisation yields dense, strong materials with unusual combinations of properties when compared with their parent glasses.⁶⁶ It is also possible to design glass-ceramics with nano- or micro-structure according to the end application.⁶⁴

A common example of glass-ceramics is apatite/wollastonite (A-W) that has improved mechanical properties than their parent glass.⁶⁷ Due to their micro-nanostructure and improved mechanical properties, these glass-ceramics could be promising matrices for bone regeneration,⁶⁸ for example, intramedullary plug in total hip replacement.⁶⁹ Surface functionalisation of glass-ceramics with lysine improved their cytocompatibility.⁷⁰

Regardless of the most obvious advantages of these bioactive glasses and ceramics, their brittle nature remains a big challenge particularly with the production of porous scaffolds. The expected reduction in strength associated with the degradation of the scaffold is also another challenge that requires careful consideration during scaffolds’ designing.

Mechanism of apatite formation

Glass composition, surface charge, types of the medium (supersaturated solutions) and test conditions are the most influencing factors that affect the nucleation of apatite onto bioactive materials.⁷¹ The mechanisms of bioactivity for various bioactive materials (such as silicate, borate glasses and some metals) have been described in detail elsewhere.^52,71–75 Among them, the mechanism of hydroxycarbonate apatite (HCA) layer formation on the surface of silicate-based glass (especially, 45S5 bioglass) implant has been most widely investigated. As the bioactivity of glasses mainly depends on the compositions of bioactive materials, a bone-bonding compositional diagram of silicate glass system (SiO₂–CaO–Na₂O–P₂O₅) has been proposed by Hench and colleagues^76,77 (as presented in Figure 1). The diagram suggested that the glasses with composition fall within the region A are bioactive and hence can induce bonding with the bone, whereas compositions in region B are nearly bioinert. Compositions in region C are highly resorbable (10–30 days) and those fall within the region D do not form glass. Therefore, selection of proper compositions which in turn regulates the surface activity of the glass materials is important to understand the mechanism of apatite formation when tested in vitro to evaluate the in vivo bone-bonding capacity of bioactive materials. For example, a comparative study between in vivo bone ingrowth and in vitro apatite formation in SBF was investigated using Na₂O–CaO–SiO₂ glass system which reported that the induction period for apatite formation on the glass surface in SBF increased with increasing SiO₂ content (from 50 to 70 mol%) which was well correlated with the results obtained from the in vivo bone ingrowth study.⁷⁸

Apatite formation on the surface of bioactive glasses occurs through a sequence of chemical reactions when immersed in SBF. A schematic illustration of the reaction sequence leading to HCA formation according to Hench and colleagues^79,80 has been described in Figure 2.⁸¹ First two stages involve the ion exchange reactions between the modifier ions of glass network (like Ca²⁺ and Na⁺) and H⁺ ions from the medium (SBF) which promote the hydrolysis of silica groups and followed by formation of silanol (Si–OH) groups on the glass surface. At stage 3, condensation and polymerisation of SiO₂-rich layer take place on the surface, whereas stage 4 implies migration of Ca²⁺ and ${PO}_{4}^{3 -}$ ions from the glass network and also from SBF medium to the SiO₂-rich layer leading to the formation of amorphous calcium phosphate (ACP) layer. At the final stage, uptake of additional ions from medium such as OH⁻, ${CO}_{3}^{2 -}$ and Na⁺ into the ACP layer promotes the conversion of ACP into HCA via crystallisation.

The release of calcium ions in combination with phosphorous ions was reported to help in deposition of apatite layer on the glass surface according to the mechanism mentioned above.^82,83 For example, large amount of Ca²⁺ ion released from CaO–SiO₂–TiO₂ glass was found to form apatite layer on its surface within a day of immersion in SBF which was suggested to be due to increase of ionic activity during the apatite nucleation process.⁸⁴ Several researchers also investigated the effect of Mg²⁺ ions on the bioactivity study and suggested that trace amounts of Mg²⁺ ions could enhance in vivo bone formation and adhesion of osteoblast cells on the glass surface.^85–91 For example, MgO content up to ~17 wt% in MgO–3CaO·P₂O₅–SiO₂ glass system was reported to promote CaP-rich layer formation and rapid mineralisation on the surface when immersed in SBF.⁹¹

Borate-based bioactive glasses (e.g. 46.1B₂O₃–24.4Na₂O–26.9CaO in mol%) follow the same mechanism of HA layer formation as described for silicate-based glasses except for the formation of SiO₂-rich layer.^73,74 The faster dissolution rate of the borate glass when compared to the silicate glass (due to their lower chemical durability) is considered as the main reason behind the fast deposition rate of HA-like material on surfaces of borate glasses.^52,74 The conversion mechanisms of borate glass into HA in phosphate solution are illustrated in Figure 3.⁷⁴ When borate glasses are immersed in a dilute phosphate solution, dissolution of Na⁺ and ${BO}_{3}^{3 -}$ ions from the glass structure into the solution occurs first. Then, ${PO}_{4}^{3 -}$ ions from the medium are assumed to react with Ca²⁺ ions leading to nucleation and growth of HA. The process is supposed to be continued until the glass is completely converted to HA.

A comparative study on bioactivity of a borate (45B5) and silicate (45S5) glasses was carried out by Liang et al.,⁹² where borate glass was found to react faster (more than five times) than silicate glasses in a solution of 0.25 M K₂HPO₄ (pH = 9). HA layer was seen to form on borate glass within 24 h, whereas HA layer was not visible on silicate glass even after 7 days of immersion in the same medium. In addition, the conversion of borate glass into HA layer was reported to be completed within 4 days (in 20 mM K₂HPO₄ solution), while silicate glass (45S5) was seen to convert to HA partially (~50%) after 70 days of test period.⁷⁴ For example, HA reaction product formed on the surface of silicate (SiO₂-46.1, CaO-26.9, Na₂O-24.4, P₂O₅-2.6 in mol%) and borate (B₂O₃-46.1, CaO-26.9, Na₂O-24.4, P₂O₅-2.6 in mol%) glasses after immersion in dilute K₂HPO₄ solution (20 mM) had a layered structure (as presented in Figure 4(a) and (b)).

Bioceramics such as wollastonite and pseudowollastonite have been revealed faster apatite formation in SBF compared to other bioglasses and glass-ceramics.^93–96 The mechanism of apatite formation on wollastonite in SBF was suggested to be due to the negative surface charge of the functional group (=Si–O–) on the ceramic surface rather than the dissolution of calcium ions into SBF.⁹⁷ Various types of sintered bioceramics such as HA and α-tricalcium phosphate (α-TCP) were seen to form apatite layer on their surface after 24 h when immersed in SBF.⁹⁸ The effect of pH (6.5 and 7.4) and the concentration of ${HCO}_{3}^{-}$ ions (4.2 and 27 mM) in modified SBF (m-SBF) were also reported to have influence on the bioactivity response of bioceramics (e.g. commercial HA Captal^®).⁹⁹ No mineralisation was detected on the HA surface when tested in m-SBF at the pH of 6.5. However, HCA layer was seen to form on the HA surface in m-SBF at pH 7.4 and a thicker HCA layer was observed on the HA substrate that immersed in m-SBF containing higher ${HCO}_{3}^{-}$ ions.

In case of metal substrate (e.g. bioinert titanium, Ti), the surface charge and the pH of the medium play the vital role in the apatite formation.^72,100,101 It has been previously reported that Ti and its alloys were found to form apatite when treated with basic (NaOH)^101,102 or acidic (H₂SO₄/HCl) solution.⁷² Ti and its alloys display a certain level of positive and negative zeta potential when exposed to a strong acidic or basic solution, followed by a subsequent heat treatment. A schematic illustration of apatite formation mechanism on the positively and negatively charged Ti metal is described in Figure 5.¹⁰⁰ When the positively charged Ti substrate is immersed into SBF, the negatively charged phosphate ions from the medium are assumed to first accumulate on its surface leading to creation of negatively charged surface. As a result, positively charged calcium ions (from medium) are migrated to the negatively charged surface in order to form a CaP layer prior to crystallisation into apatite layer, as shown in Figure 5(a). On the other hand, the Ti surface is expected to form a sodium titanate layer after alkali (in NaOH) and heat treatments. Afterwards, the sodium titanate exchanges Na⁺ ions with the H₃O⁺ ions (from SBF) to form Ti–OH on the surface which leads to an increase in pH due to the consumption of H₃O⁺ ions from medium and releasing Na⁺ ions to the medium. Consequently, Ti metal that carries a negative charge would initially attract calcium cations followed by phosphate anions to form CaP layer, as shown in Figure 5(b). Therefore, Ti–OH on the surface seems to induce apatite nucleation.^101,103 For example, the bone-like apatite layer was reported to form on the NaOH (5M) and heat (600°C for 1 h)-treated Ti metal after immersion in SBF for 10 days. Likewise, alkali-treated titanium-based alloys such as Ti-6Al-4V, Ti-6Al-2Nb-Ta and Ti-15Mo-SZr-3Al were also reported to promote bone-like apatite deposition on their surfaces in SBF following the same mechanism (see Figure 5).^102,104 Apatite formation on the surface of titanium has also been enhanced after acid treatment^105,106 as well as by producing a negative surface charge via the light illumination of SBF with mercury lamp.⁸ Ti substrates without any treatment possess no surface charge; therefore, when exposed to SBF, they can only form a CaP layer on their surface which does not bond to the bone.¹⁰⁷

Phosphate-based glasses (PBGs) have attracted huge interest in the field of biomaterials and tissue engineering due to their chemical similarity with the inorganic component of the natural bone and controllable degradation profile.¹⁰⁸ However, very few literatures have examined the bioactivity of PBGs.^109–112 The presence of TiO₂ in phosphate glasses is reported to induce CaP nucleation and improve their bioactivity.¹¹³ Ti-doped PBGs were investigated for bioactivity and an intermediate hydrated titania layer (0.5–2 µm Ti–OH layer/gel) was observed to form in SBF which played an important role in the formation of apatite.¹¹¹ Another bioactivity study on xCaO–(90-x)P₂O₅–10TiO₂ glasses was suggested that the formulations containing 35 and 40 mol% of P₂O₅ did not support any apatite layer deposition even after 30 days of immersion in SBF. On the other hand, apatite layer was found to form on the phosphate invert glasses, containing 30 mol% of P₂O₅, after 20 days of immersion in SBF.¹¹⁴ This was suggested to be due to the release of relatively small amount of phosphate ions from lower P₂O₅ containing glasses which promoted the apatite nucleation.¹⁰⁹

Apatite formation also depends on the basicity of the gel layer formed on the glasses and the amount of the functional groups present for HA nucleation in the layer.¹¹⁴ The basicity of the gel layer can be enhanced via the addition of Na₂O.¹⁰⁹ The high amount of Na₂O and CaO as well as the relatively higher ratio of CaO/P₂O₅ would provide highly reactive surface of bioactive glasses in physiological environment which would eventually facilitate apatite formation.¹¹⁵ Hydrated gel layers such as Si–OH, Ti–OH, Ta–OH, Zr–OH, Nb–OH, –COOH and PO₄H₂ groups are proved to provide nucleation sites for HA in SBF.¹¹⁶

Apart from apatite formation, several other possible phases of calcium orthophosphates such as ACP, brushite CaHPO₄·2H₂O (DCPD), monetite CaHPO₄(DCPA) or octacalcium phosphate (OCP) can be formed in SBF depending on the experimental conditions of formation and state of ageing.¹¹⁷ These calcium orthophosphates except ACP are more stable than HA in acidic conditions¹¹⁸ and at the later stages, all of the phases would be converted into HA in various pathways.¹¹⁹

Test protocols for in vitro bioactivity experiment

In vivo bioactivity of a material can be predicted from its ability to form apatite in SBF and/or other similar types of supersaturated medium.⁶ A vast number of work have been focused so far on the effect of composition and morphology of glass and ceramic materials on their in vitro dissolution rate and apatite-forming ability. Various protocols with respect to the types of medium (e.g. SBF, phosphate-buffered saline (PBS), TRIS, K₂HPO₄, Dulbecco’s Modified Eagle’s Medium (DMEM)), morphology of materials (powder, pellets, discs), surface area to volume ratio and the test condition (static, dynamic) have been considered for the in vitro bioactivity experiment (summarised in Table 1).

Table 1.

Parameters and test protocols that have been used to investigate the bioactivity of various biomaterials.

Materials	Medium	Geometry	Surface area or mass/vol	Condition	Length of study	Comments	Ref
Silicate glass (45S5)	SBF	Block (1 × 1.5 × 0.2 cm³)	S/V = 0.05 cm⁻¹	Static	14 days	HA formed (2 days)	Helebrant et al.¹²⁰
Glass-ceramics (A-W)	SBF	Block (22 × 40 × 2 mm³)	200 mL	–	30 days	HA formed (7 days)	Kokubo et al.¹²¹
Silicate glass (45S5/S53P4/S68)	SBF/TRIS/Na-PBS	Block (20 × 15 × 1.5 mm³)	20 mL S/V = 0.4 cm⁻¹	Solution was replenished after 7 days	14 days	CaP layer formed on 45S5/S53P4 (24 h) and S68 (7 days)	Varila et al.¹²²
Borate glass 15Na₂O–15CaO–xB₂O₃–(70-x)P₂O₅	SBF	Particles (106–180 µm)	500 mg in 50 mL	–	30 days	HA formation increased with increasing B₂O₃ content	Abo-Naf et al.¹²³
Silicate glass (37CaO–58SiO₂–5P₂O₅)	SBF	Powder (<20 mm particles)	600 mg in 1 L	Stirring (100 r/min)	30 days	HCA formed (24 h)	Turdean-Ionescu et al.¹²⁴
Silicate glass (46S6)	SBF	Disc (13 mm diameter × 5 mm thick)	30 mL	Agitation	30 days	HA formed (1 day)	Bui et al.¹²⁵
Bioceramics (A-W; BG; HA; HA/TCP; α-TCP; β-TCP)	SBF	Cube (5 × 5 × 5 mm³)	200 mL	–	–	OCP formed (1 day) on all bioceramics except on β -TCP	Xin et al.⁹⁸
Calcium aluminate (CA), glass ionomer cement (GIC), CA/GIC hybrid	PBS	Block (22 × 15 × 4 mm³)	50 mL	PBS was changed once a week	28 days	HA formed on CA (24 h) and CA/GIC hybrid (7 days) GIC did not show any bioactivity in PBS	Lööf et al.¹²⁶
Silicate glass 41.7SiO₂-(44.14-X) CaO-XMgO-3.13ZnO-5.2Na₂O-K₂O-4.7P₂O₅	SBF and Tris-buffer	Powder (<45 μm)	75 mg in 50 mL	Agitated using mechanical shaker	30 days	HA formed on non-magnesium containing glasses by 7 days in both SBF and Tris-buffer whereas HA formed on Mg containing glasses after 1 month in SBF but not in Tris-buffer	Al-Noaman et al.¹²⁷
Bioceramics (HA)	SBF	Particles (<5 mm)	50 mg in 120 mL	–	120 h	HA formed (12 h)	Kim et al.¹²⁸
Glass-ceramics (A-W)	SBF	Block (22 × 40 × 2 mm³)	200 mL	–	60 days	HA formed (7 days)	Kokubo et al.¹²⁹
Glass-ceramic (Ceravital)	SBF	Block (15 × 10 × 1 mm³)	35 mL	–	20 days	HCA formed (1 day)	Ohtsuki et al.¹³⁰
Borate glass (45B5)	K₂HPO₄ (0.25 M)	Disc (15-mm diameter and 3-mm thick)	–	–	14 days	HA formed (1 day)	Liang et al.⁹³
Borate glass (36–61 mol% B₂O₃)	SBF/K₂HPO₄ (0.25 M)	Powder (25−75 μm)	1.5 mg/mL ratio	Gentle agitation	7 days	HCA formed (6 h)	Lepry and Nazhat¹³¹
Borate glass	K₂HPO₄ (0.2 M)	Disc (5-mm diameter × 5-mm thick)	100 mL	Static	7 days	HA formed (6 days)	Liang et al.¹³²
Silicate glass (58S)	DMEM	Particles (20–40 µm)	75 mg in 50 mL	Solution was changed at 6 h, 24 h, and 2 days	3 days	HCA formed (3 days)	Theodorou et al.¹³³
Titanium alloy (Ti6Al4V)	DMEM	Block (10 × 10 × 1 mm³)	40 mL	–	360 h	HA formed (360 h)	Faure et al.¹³⁴
ISO/23317:2014(E)	SBF	Disc (10-mm diameter × 2-mm thick). Block (10 × 10 × 2 mm³)	Vs = Sa/10 mL ratio	Static	30 days	Apatite formation	ISO 23317:2014¹³⁵
Unified method (TC04)	SBF	Particles (45–90 µm)	75 mg in 50 mL	Agitation (120 r/min)	28 days	Apatite formation	Maçon et al.¹³⁶

SBF: simulated body fluid; HA: hydroxyapatite; PBS: phosphate-buffered saline; CaP: calcium phosphate; HCA: hydroxycarbonate apatite; DMEM: Dulbecco’s Modified Eagle’s Medium; OCP: octacalcium phosphate; TCP: tricalcium phosphate.

The effect of different solutions on dissolution of bioactive materials has been studied.^137–141 In the early 1980s, TRIS buffer was used to evaluate the apatite-forming ability of glass and glass-ceramic materials.^142–144 Later, in 1990, Kokubo et al.¹²¹ developed the simulated solution which reproduced in vivo surface structure changes of glass-ceramics A-W more precisely than TRIS. The pH of simulated solutions is maintained using TRIS of HEPES buffers. However, these buffers were seen unable to maintain the neutral pH of SBF during in vitro test.¹⁴⁰ SBF is a supersaturated solution containing similar ionic concentrations of inorganic parts of human blood plasma (presented in Table 2).¹⁴⁵ However, it has higher Cl⁻ ions and lower ${HCO}_{3}^{-}$ ion concentration than those of the blood plasma. In 2001, Helebrant et al.¹²⁰ investigated the apatite formation on 45S5 bioglass using a series of SBF solutions with increasing ${HCO}_{3}^{-}$ ion concentration up to the value close to blood plasma and suggested that the SBF with increased amount of ${HCO}_{3}^{-}$ ions is more appropriate for in vitro bioactivity testing of biomaterials. Oyane et al.¹⁴⁶ also prepared the revised SBF (r-SBF) and modified SBF (m-SBF) which contained the ion concentrations equal or close to those in blood plasma (except for ${HCO}_{3}^{-}$ ion concentrations in m-SBF).¹⁴⁷ In terms of stability, r-SBF and m-SBF were seen to remain stable, no change in ion concentrations and pH value, up to 2 and 8 weeks, respectively, when stored in sealed containers at 36.5°C.

Table 2.

Ionic concentration in human blood plasma in comparison with various developed SBF medium.¹⁴⁵

Ion	Human blood plasma (pH 7.2–7.4)	Ion concentration (10⁻³ mol) in
Ion	Human blood plasma (pH 7.2–7.4)	SBF (pH 7.4)	Revised-SBF (r-SBF)	Modified-SBF (m-SBF)
Na⁺	142.0	142.0	142.0	142.0
K⁺	5.0	5.0	5.0	5.0
Mg²⁺	1.5	1.5	1.5	1.5
Ca²⁺	2.5	2.5	2.5	2.5
Cl⁻	103.0	147.8	103.0	103.0
${HCO}_{3}^{-}$	27.0	4.2	27.0	10
${HPO}_{4}^{2 -}$	1.0	1.0	1.0	1.0
${SO}_{4}^{2 -}$	0.5	0.5	0.5	0.5

SBF: simulated body fluid.

In addition to SBF, cell culture medium (DMEM) were also used for bioactivity testing, and it has been found that the non-buffered DMEM solution containing an organic phase was not suitable for bioactivity test.¹⁴⁸ DMEM contains lower concentration of Ca²⁺ ions but higher concentration of ${HCO}_{3}^{-}$ ions compared to blood plasma which leads to formation of CaCO₃ instead of apatite.¹⁴⁸ However, recently Popa et al.¹⁴⁹ studied the in vitro bioactivity of BG films (SiO₂ 38.5, CaO 36.1, P₂O₅ 5.6, MgO 15.2, ZnO 4, and CaF₂ 0.6 in mol%) in different medium (namely SBF, DMEM, DMEM supplemented with 10% foetal bovine serum) and found that bioactivity test in DMEM supplemented with proteins under homeostatic conditions was more appropriate than that in SBF. They also suggested an unique bioactivity testing protocol utilising specific surface area to medium volume ratio (Sa/V = 0.5 cm²/mL) for the materials with different shapes and dimensions including bulk objects, thin films, powder and scaffolds.¹⁴⁹

PBS medium was also used in in vitro studies for bioactive glass (45S5) containing polymer (poly-l-lactic acid, PLLA and polylactic-co-glycolic acid, PLGA) composites.^150,151 It was found that the formation of apatite on the glass surface was faster in PBS than SBF or TRIS.¹³⁹ Fagerlund et al.¹³⁹ investigated the dissolution of bioactive glasses (45S5, S53P4, 13-93) in PBS and reported that pH of the solution increased when alkaline and alkaline earth ions dissipated from the glasses. The release of silica ions and CaP precipitation also increased at higher pH. They also found that the CaP layer formed quickly due to the higher concentration of phosphorus ions in PBS.¹³⁹ In vitro bioactivity of glasses especially for borate glasses has been evaluated in aqueous K₂HPO₄ medium.^{57,92,131,152} This medium was used to save the experimental time through the reaction of available ${HPO}_{4}^{2 -}$ and OH⁻ ions in K₂HPO₄ solution with glasses.⁹²

Apart from the use of various media, other factors such as geometry of the test specimen, surface area to volume ratio and test conditions are also key to justify the test protocol. Therefore, an ISO standard (ISO/23317:2014(E): Implants for surgery – In vitro evaluation for apatite-forming ability of implant materials) has been proposed to conduct the in vitro bioactivity test.¹³⁵ The ISO standard suggested to use acellular and protein-free SBF solution buffered with TRIS. The standard also suggested the dimension and shape of test specimen only for bulk compact inorganic materials (solid disc and rectangular block) with a defined sample surface area to SBF volume ratio (V_SBF = 100 × S_a; where V_SBF is the volume of SBF and S_a is the surface area of glass). However, this ratio is not defined for the materials in other forms of biomaterials such as powder and porous scaffolds. Moreover, the ISO standard stated the static testing condition, whereas some literatures conducted dynamic condition to mimic the in vivo environment.^153–156

Recently, the members of the Technical Committee 4 (TC04) of the International Commission on Glass (ICG) have been proposed unified method (modified version of ISO standard) for testing the bioactivity of glass particles (45–90 μm), particularly those of high surface area.¹³⁶ The modified method suggested use of fixed mass per solution volume ratio (75 mg in 50 mL) with agitation (120 r/min).

Biomedical applications of CaP

Earlier in section ‘Mechanism of apatite formation’, it has been mentioned that during the initial stage of the in vitro bioactivity study, the ACP layer formed onto the surface of bioactive materials followed by crystallisation into apatite (HA), which is nucleated by the interaction of ions and pH of the medium. However, some biomaterials can be limited to release of the desirable ions (due to their compositions) and also may be unable to produce favourable pH environment for ACP layer to be crystallised into apatite at the late stages of the in vitro study. Therefore, the biomedical applications of both ACP and apatite will be discussed in this section.

Similar to apatite (i.e. HA), ACPs have excellent biological, osteoconductivity and no cytotoxicity responses; therefore, they have been introduced to orthopaedics and dentistry.¹⁵⁷ ACPs have been investigated for a range of biomedical applications in different forms: powders, granules, composites, self-setting cements or coatings.¹⁵⁸ Examples of biomedical applications of CaP-based materials can be seen in Figure 6.

Dental applications

Due to good osteoconductivity and tuneable degradation rate of ACPs, they have been added to mouthwashes, chewing gums, toothpastes and also to ionomer cements as a filler for carious lesions. Complexes of casein phosphopeptides (CPP) and ACP have been used as abrasive pastes for treatment of tooth sensitivity after root canal repair, scaling or bleaching procedures. Clinical trial revealed an increase in the content of inorganic phosphate and calcium in supragingival plaque after 3 days’ use of mouthwash containing CPP-ACP complexes. This product is commercially known as GC Tooth Mousse. CPP-ACP complexes can be also incorporated into food, drinks and confectionary for potential prevention of the dental caries due to their natural origin (milk derivative). ACP has also been explored as a filler for bioactive polymer composites to be used for tooth repair. Tooth repair can be stimulated through the released ions (calcium and phosphate) and form the breakdown of ACP particles. Effect of ACP addition to orthodontic adhesives has also been evaluated and demonstrated satisfactory bracket bonding strength in clinical trials.¹⁵⁷ Toothpastes containing CaP can be used to reduce tooth sensitivity, to promote remineralisation of the demineralised enamel and for whitening and polishing purposes. Toothpastes containing HA have shown a significant positive effect on sensitivity and whitening of tooth, and it was found that the whitening effect increased as the amount of HA within the toothpaste increased. Both ACP and HA have been added to toothpaste and are available commercially. Examples of HA-containing toothpastes are Sensitive Reminx (Pharma Jenistec Co., Ltd, Korea), Triple Denta (TripleLife Co., Ltd, Korea), Kalident – calcium hydroxyapatite (Kalichem Italia S.r.l.), DIO (DIO Co., Ltd, Korea), Coolin Bubble (Canavena Co., Ltd, Korea) and YP Dental (You Co., Ltd, Japan). There are also toothpastes containing ACP such as Enamelon, Arm and Hammer’s Enamel Care and Premier Dental’s Enamel Pro.¹⁶⁰

Bone repair

Human bone contains 70% of CaP minerals; hence, CaP-based materials have been considered as the best choice for repairing the damaged bone post trauma.¹⁶¹ CaP has been thoroughly studied in various forms for repairing hard tissue because of their excellent biocompatibility, composition similarity with the bone mineral, inexpensiveness and easy to produce.¹⁶¹ It has been reported that the rate of new bone formation was well correlated with the rate of ACP resorption. Moreover, ACP revealed significantly better osteoconductivity response compared to TCP in vivo study. Therefore, ACP has been incorporated within biodegradable polymers (e.g. PLLA, PLGA) for manufacturing porous scaffolds for bone and cartilage tissue engineering. It was also found that ACP particles within the polymer composites transferred after a short period of immersion in PBS into bone-like apatite which would potentially facilitate the formation of new bone in vivo and clinical trials.¹⁵⁷ Recently, CaP powder was manufactured into three-dimensional (3D) porous scaffolds with the aid of additive manufacturing techniques (robocasting; see Figure 7).

Bioactive coatings

Metallic implants are still commonly used for load-bearing applications such as hip-joint replacement, bone fixation devices (i.e. nails, plates and screws) and tooth sockets.¹⁵⁸ The lack of the bioactivity and poor bonding with the host bone of the metals had been a challenge for their clinical use. Therefore, coating with bioactive materials such as ACP, HA or other CaPs have been conducted to overcome the bioactivity and biocompatibility complications. HA coating of metallic implants was found to enhance their rate of clinical success to be more than 98%.¹⁶³ Numerous coating methods have been utilised, for example, thermal spray, plasma spraying, electrophoretic and biomimetic deposition.¹⁶⁴ The presence of ACP was reported in plasma-sprayed HA coating; however, the quantity could not be well controlled.¹⁵⁸

Since magnesium alloys are biodegradable metals, non-toxic and have similar mechanical properties of the cortical bone, they have been considered as attractive candidates for load-bearing biomedical applications. The main complication associated with the use of magnesium implant is the fast degradation in physiological environment. Thus, CaP coatings have been applied to magnesium alloys to enhance their bioactivity, biocompatibility and control their degradation rates. A significant reduction in degradation rates of magnesium alloys was obtained after surface coating with CaP materials.¹⁶⁵

Drug and gene delivery

CaP-based nanoparticles have been explored for targeted drug and gene delivery due to their unique characteristics; similarity to inorganic component of bone, excellent adsorption capability to many biomolecules and proteins and biodegradability in moderate acidic medium (similar to the pH inside lysosome). CaP nanoparticles, with size less than 200 nm, can enter into the cells via endocytosis mechanism and may end up in lysosomes. Consequently, CaP can break down at acidic conditions into phosphate and calcium ions. Phosphate ions are not harmful and amount of calcium ions can be tolerated using moderate quantities of the nanoparticles. Dissolution of CaP nanoparticles after cellular uptake made them a promising competitor to conventional nanoparticles (silica, gold and polymers). CaP nanoparticles loaded with vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs) were produced in a water-based paste for bone repair purposes through direct injection into the defect (see Figure 8(a)).¹⁶¹ CaP nanoparticles can also be used to deliver drugs and biomolecules by producing them with multi-shells architecture (see Figure 8(b)).

Soft tissue engineering

Due to their ease of preparation into fibres, CaP glass fibres (in particular, phosphate based) were studied for their potential use in muscle and nerve regeneration. A 3D fibrous construct, having the composition of (P₂O₅)_62.9(Al₂O₃)_21.9ZnO_15.2, supported the proliferation and differentiation of human masseter muscle–derived cell cultures.⁹ CaP glass fibres, based on (P₂O₅)₅₀(CaO)₃₀(Na₂O)_20-x(Fe₂O₃)_x composition where x = 1–5, supported high level of attachment of immortal muscle precursor cell line.³⁶

Phosphate glass fibres containing 5–22.5 wt% Fe₂O₃ have been used as reinforcing agents in the development of bioabsorbable composites designed for orthopaedic applications. A cortical plug method was used to test the biocompatibility of these glasses; the results showed that no inflammation was observed over periods of up to 5 weeks.¹⁶⁸ Due to the intriguing ability of phosphate glass fibres to form capillary-like channels during their degradation,⁴⁷ they were used for in situ formation of continuous aligned channels of 30–40 μm in diameter within 3D dense collagen scaffolds to allow for proper diffusion of nutrients and waste products through the constructs.⁵¹ These constructs therefore maintained an excellent viability of human oral fibroblasts that formed a 3D network.⁵¹

Summary

This article aimed to review the various types of biomaterials (i.e. silicate-, borate- and phosphate-based glasses; glass-ceramics; bioceramics; and metals) investigated for in vitro bioactivity evaluation. CaP formation route on the surface of these biomaterials are dependent on the surface activity of the materials (in test medium) and test environment (i.e. ionic concentrations of medium and pH). Materials geometry (i.e. powder, pellets, discs, cubes, blocks), material to medium volume ratio and experimental condition (static or dynamic) are also crucial factors that require consideration during bioactivity test. For example, ISO 23317:2014(E) standard suggests the sample surface area (solid disc/block) to SBF volume ratio (V_SBF = 100 × S_a) to be used at static condition, whereas recently a unified method (modified form of ISO) has been suggested to use of fixed mass (45–90 μm particles) per medium volume ratio (75 mg in 50 mL) with agitation during the bioactivity testing. Apart from these various test parameters, adequate physico-chemical characterisation is necessary to draw conclusive understanding of the nature of deposited CaP phase. Although plenty of ACPs and bone-like apatite materials have already shown their potential in biomedical applications (dental, bone repair, gene and drug delivery) and also some of them are already available in the market, some factors such as possible experimental mistakes including evolution of the substrate in medium, contamination within medium by microorganisms and residual presence of precursor phases are responsible for experimental failure of apatite formation when investigating a new bioactive material, which still warrant further research and validation.

Footnotes

Declaration of conflicting interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The author (M.T.I) would like to acknowledge the financial support provided by the University of Nottingham, Faculty of Engineering (the Dean of Engineering Research Scholarship for International Excellence).

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