Sudden infant death syndrome (SIDS) has been associated with the volatilization of arsenic, antimony or phosphorus compounds from infants' bedding material by micro-organisms, the so-called ‘toxic gas hypothesis’. The volatilization of arsenic by aerobic micro-organisms isolated from new sheepskin bedding material, as well as on material used by a healthy infant and by an infant who perished of SIDS, was examined. Three fungi were isolated from a piece of sheepskin bedding material on which an infant perished of SIDS, which methylated arsenic to form trimethylarsenic(V) species, precursors to volatile trimethylarsine. These three fungi were identified as Scopulariopsis koningii, Fomitopsis pinicola and Penicillium gladioli by their 26S-ribosomal RNA polymerase chain reaction products. These fungi were not previously known to methylate arsenic. The volatilization of arsenic by these three fungi was then examined. Only P. gladioli volatilized arsenic and only under conditions such that the production of sufficient trimethylarsine to be acutely toxic to an infant is unlikely. S. brevicaulis grew on the sheepskin bedding material and evolved a trace amount of trimethylarsine. Known human pathogens such as Mycobacterium neoaurum and Acinetobacter junii were isolated from used bedding.
Bentley R . Bartolomeo Gosio, 1863-1944: an appreciation . Adv Appl Microbiol2000; 48: 229-250 .
2.
Sanger CR . On chronic arsenical poisoning from wallpapers and fabrics . Proc Am Acad Arts Sci1893; 29: 112-147 .
3.
Challenger F , Higgenbottom C , Ellis L . Formation of organometal-loidal compounds by microorganisms. 1. Trimethylarsine and dimethylethylarsine. J Chem Soc1933; 95-101.
Cullen WR , Reimer KJ . Arsenic speciation in the environment . Chem Rev1989; 89: 713-764 .
6.
Wickenheiser EB , Michalke K , Drescher C , Hirner AV , Hensel R . Development and application of liquid and gas-chromatographic speciation techniques with element specific (ICP-MS) detection to the study of anaerobic arsenic metabolism . Fresenius J Anal Chem1998; 362: 498-501 .
7.
Cox DP , Alexander M . Production of trimethylarsine gas from various arsenic compounds by three sewage fungi . Bull Environ Contam Toxicol1973; 9: 84-88 .
8.
Richardson BA . Cot mattress biodeterioration and SIDS . Lancet1990; 335: 670-670 .
9.
Turner Report. Sudden Infant Death Syndrome. London: HMSO , 1991.
10.
Richardson BA . Sudden Infant Death Syndrome: a possible primary cause . J Forensic Sci Soc1994; 34: 199-204 .
11.
Howatson AG , Patrick WJ , Fell GS , Lyon TD , Gibson AA . Cot mattresses and Sudden Infant Death Syndrome . Lancet1995; 345: 1044-1045 .
12.
Limerick Report. Expert group to investigate cot death theories: toxic gas hypothesis. London: HMSO , 1998.
13.
Andrewes P , Cullen WR , Feldmann J , Koch I , Polishchuk E , Reimer KJ . The production of methylated organoantimony compounds by Scopulariopsis brevicaulis . Appl Organomet Chem1998; 12: 827-842 .
14.
Jenkins RO , Craig PJ , Goessler W , Miller D , Ostah N , Irgolic KJ . Biomethylation of inorganic antimony compounds by an aerobic fungus: Scopulariopsis brevicaulis . Environ Sci Technol1998; 32: 882-885 .
15.
Warnock DW , Delves HT , Campbell CK , Croudace IW , Davey KG , Johnson EM . Toxic gas generation from plastic mattresses and Sudden Infant Death Syndrome . Lancet1995; 346: 1516-1520 .
16.
Kelley J , Allsopp D , Hawksworth DL . Sudden Infant Death Syndrome (SIDS) and the toxic gas hypothesis: microbiological studies of cot mattresses . Hum Exp Toxicol1992; 11: 347-355 .
17.
Mitchell EA , Thompson JMD , Ford RPK , Taylor BJ . Sheepskin bedding and the Sudden Infant Death Syndrome . J Pediatr1998; 133: 701-704 .
18.
Raab A , Hansen HR , Zhuang L , Feldmann J . Arsenic accumulation and speciation analysis in wool from sheep exposed to arsenosugars . Talanta2002; 58: 67-76 .
19.
Burrows GJ , Turner EE . Experiments on the production of compounds containing arsenic as a center of optical activity . J Chem Soc1921; 119: 426-437 .
20.
Merijanian A , Zingaro RA . Arsine oxides . Inorg Chem1966; 5: 187-191 .
21.
Bondgaard M , Nørum U , Bjerregaard P . Cadmium accumulation in female shore crab Carcinus maenas during the moult cycle and ovarian maturation . Mar Biol2000; 137: 995-1004 .
22.
Granchinho SCR , Polishchuk E , Cullen WR , Reimer KJ . Biomethylation and bioaccumulation of arsenic(V) by marine alga Fucus Gardneri . Appl Organomet Chem2001; 15: 553-560 .
23.
Cullen, WR , Li H , Hewitt G , Reimer KJ , Zalunardo N . Identification of extracellular arsenical metabolites in the growth medium of the microorganisms Apiotrichum humicola and Scopulariopsis brevicaulis . Appl Organomet Chem1994; 8: 303-311 .
24.
Kurtzman CP , Robnett CJ . Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene . J Clin Microbiol1997; 35: 1216-1223 .
25.
Lane DJ . 16S/23S rRNA sequencing. In Stackebrandt E , Goodfellow M eds. Nucleic acid techniques in bacterial systematics. Chichester: Academic Press , 1991: 115-175.
26.
O'Donnell K . Fusarium and its near relatives. In Reyholds DR , Taylor JW eds. The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. Wallingford: CAB International , 1993: 225-233.
27.
Cox DP , Martin A . Effect of phosphate and other anions on trimethylarsine formation by Candida humicola . Appl Microbiol1973; 25: 408-413 .
28.
Andrewes P , Cullen WR , Polishchuk E . Confirmation of the aerobic production of trimethylstibine by Scopulariopsis brevicaulis . Appl Organomet Chem1999; 13: 659-664 .
29.
Feldmann, J . Summary of a calibration method for the determination of volatile metal(loid) compounds in environmental gas samples by using gas chromatography-inductively coupled plasma-mass spectrometry . J Anal Atomic Spectrom1997; 12: 1069-1076 .
30.
Kolacz R , Bodak E , Dobrzanski Z , Patkowska-Sokola B . Trace elements in the wool of Polish Merino sheep grazed in polluted and unpolluted environment . Czech J Anim Sci1999; 44: 509-514 .
31.
Chin JC , Watts JE . Relationship between the immune response of sheep and the population dynamics of bacteria isolated from ‘eece rot lesions . Vet Microbiol1992; 32: 63-74 .
de Beaufort A J , Bernards AT , Dijkshoorn L , van Boven CP . Acinetobacter junii causes life-threatening sepsis in preterm infants . Acta Paediatr1999; 88: 772-775 .
34.
Marchisio VF , Fusconi A . Morphological evidence for keratinolytic activity of Scopulariopsis spp. isolates from nail lesions and the air . Med Mycol2001; 39: 287-394 .
35.
Andrewes P , Cullen WR , Polishchuk E . Arsenic and antimony biomethylation by Scopulariopsis brevicaulis: interaction of arsenic and antimony compounds . Environ Sci Technol2000; 34: 2249-2253 .
Bird ML , Challenger F , Charlton PT , Smith JO . Studies on biological methylation II. The action of moulds on inorganic and organic compounds of arsenic . Biochem J1948; 43: 78-83 .
38.
Kaul S , Sumbali G . Keratinolysis by poultry farm soil fungi . Mycopathologia1997; 139: 137-140 .
39.
Malviya HK , Rajak RC , Hasija SJ . Synthesis and regulation of extracellular keratinase in three fungi isolated from the grounds of a gelatin factory, Jabalpur, India . Mycopathologia1992; 120: 1-4 .
40.
Sankov EA , Suchkova GG , Andreeva KI . Biological damage to wool fibers as determined by dyeing. Izv Vyssh Ucheb Zaved, Technol Tekst Prom1972; 154-155.
41.
Yamauchi H , Kaise T , Takahashi K , Yamamura Y . Toxicity and metabolism of trimethylarsine in mice and hamsters . Fundam Appl Toxicol1990; 14: 399-407 .
42.
Irvin TR , Irgolic KJ . In vitro prenatal toxicity of trimethylarsine, trimethylarsine oxide and trimethylarsine sulfide . Appl Organomet Chem1995; 9: 315-321 .
43.
Kinney HC , Filiano JJ , Sleeper LA , Mandell F , ValdesDapena M , White WF . Decreased muscarinic receptor binding in the arcuate nucleus in Sudden Infant Death Syndrome . Science1995; 269: 1446-1450 .
44.
Okado N , Narita M , Narita N . A serotonin malfunction hypothesis by finding clear mutual relationships between several risk factors and symptoms associated with Sudden Infant Death Syndrome . Med Hypotheses2002; 58: 232-236 .
45.
Fifer WP , Myers MM . Sudden fetal and infant deaths: shared characteristics and distinctive features . Semin Perinatol2002; 26: 89-96 .
46.
Harper RM , Kinney HC , Fleming PJ , Thach BT . Sleep influences on homeostatic functions: implications for Sudden Infant Death Syndrome . Respir Physiolo2000; 119: 123-132 .
47.
Franco P , Szliwowski H , Dramaix M , Kahn A . Decreased autonomic responses to obstructive sleep events in future victims of Sudden Infant Death Syndrome . Pediatr Res1999, 46: 33-39 .
48.
Herschkowitz N , Kagan J , Zilles K . Neurobiological bases of behavioural developments in the first year . Neuropediatrics1997; 28: 296-306 .
