Abstract
Pulsed laser desorption is used as a means of volatilizing nonvolatile and thermally labile molecules for entrainment into a supersonic jet expansion. The jet expansion provides ultracold molecules whose sharp spectral features are probed by resonant two-photon ionization spectroscopy in a time-of-flight mass spectrometer. Such jet-cooled spectra are demonstrated for tyrosine and related structural analogs. Despite the similarity between these tyrosine-based compounds, electronic spectroscopy is shown to be a sensitive probe of small structural changes in these related biological compounds.
Get full access to this article
View all access options for this article.
References
1.
Lubman
D. M.
, Anal. Chem. 59 , 31A (1986 ).
2.
Klimcak
C.
and
Wessel
J.
, Anal. Chem. 52 , 1283 (1980 ).
3.
Rettner
C. T.
and
Brophy
J. H.
, Chem. Phys. 24 , 89 (1981 ).
4.
Zandee
L.
and
Bernstein
R. B.
, J. Chem. Phys. 70 , 2574 (1979 ).
5.
Lichtin
D. A.
Datta-Ghosh
S.
Newton
K. R.
, and
Bernstein
R. B.
, Chem. Phys. Lett. 75 , 214 (1980 ).
6.
Lubman
D. M.
and
Kronick
M. N.
, Anal. Chem. 54 , 660 (1982 ).
7.
Tembreull
R.
and
Lubman
D. M.
, Anal. Chem. 56 , 1962 (1984 ).
8.
Irion
M. P.
Bowers
W. D.
Hunter
R. L.
Rowland
F. S.
, and
McIver
R. T.
Jr.
Chem. Phys. Lett. 93 , 375 (1982 ).
9.
Carlin
T. J.
and
Freiser
B. S.
, Anal. Chem. 55 , 1955 (1983 ).
10.
Boesl
U.
Neusser
H. J.
, and
Schlag
E. W.
, Chem. Phys. 55 , 193 (1981 ).
11.
Dietz
T. G.
Duncan
M. A.
Liverman
M. G.
, and
Smalley
R. E.
, J. Chem. Phys. 73 , 4816 (1980 ).
12.
Sack
T. M.
McCrery
D. A.
, and
Gross
M. L.
, Anal. Chem. 57 , 1290 (1985 ).
13.
Syage
J. A.
and
Wessel
J. E.
, J. Chem. Phys. 85 , 6806 (1986 ).
14.
Dobson
R. L. M.
D'Silva
A. P.
Weeks
S. J.
, and
Fassel
V. A.
, Anal. Chem. 58 , 2129 (1986 ).
15.
Levy
D. H.
Wharton
L.
, and
Smalley
R. E.
, Acc. Chem. Res. 10 , 139 (1977 ).
16.
Amirav
A.
Even
U.
, and
Jortner
J.
, J. Chem. Phys. 71 , 2319 (1979 ).
17.
Imasaka
T.
O'Kamura
T.
, and
Ishibashi
N.
, Anal. Chem. 58 , 2152 (1986 ).
18.
Pepich
B. V.
Callis
J. B.
Danielson
Sheldon J. D.
, and
Gouterman
M.
, Rev. Sci. Instrum. 57 , 878 (1986 ).
19.
Hayes
J. M.
, Chem. Rev. 87 , 745 (1987 ).
20.
Posthumus
M. A.
Kistemaker
P. G.
Meuzelaar
H. L. C.
, and
Ten de Brauw
Noever M. C.
, Anal. Chem. 50 , 985 (1978 ).
21.
Conzemius
R. J.
and
Capellan
J. M.
, Int. J. Mass Spectrom. Ion Phys. 34 , 197 (1980 ).
22.
Tembreull
R.
and
Lubman
D. M.
, Anal. Chem. 59 , 1003 (1987 ).
23.
Gentry
W. R.
and
Giese
C. F.
, Rev. Sci. Instrum. 49 , 595 (1978 ).
24.
Rorden
R. J.
and
Lubman
D. M.
, Rev. Sci. Instrum. 54 , 641 (1983 ).
25.
Tembreull
R.
and
Lubman
D. M.
, Anal. Chem. 58 , 1299 (1986 ).
26.
Grotemeyer
J.
Boesl
U.
Walter
K.
, and
Schlag
W.
, Org. Mass Spectrom . 21 , 645 (1986 );
Engelke
F.
Hahn
J. H.
Henke
W.
Zare
R. N.
, Anal. Chem. 59 , 909 (1987 ).
27.
Lubman
D. M.
Rettner
C. T.
, and
Zare
R. N.
, J. Phys. Chem. 86 , 1129 (1982 ).
28.Courtesy of Spectra Physics-Report on Design of the EXC-1 Excimer Laser System .
29.
Burger
A.
, A Guide to the Chemical Basis of Drug Design (John Wiley & Sons , New York , 1983 ).
30.
Tembreull
R.
and
Lubman
D. M.
, Appl. Spectrosc. 41 , 431 (1987 ).
31.
Li
L.
and
Lubman
D. M.
, Anal. Chem. 59 , 2538 (1987 ).
32.
Oikawa
A.
Abe
H.
Mikami
N.
, and
Ito
M.
, J. Phys. Chem. 88 , 5181 (1984 ).
33.
Dunn
T. M.
Tembreull
R.
, and
Lubman
D. M.
, Chem. Phys. Lett. 121 , 453 (1985 ).
34.
Park
Y. D.
Rizzo
T. R.
Peteanu
L. A.
, and
Levy
D. H.
, J. Chem. Phys. 84 , 6539 (1986 ).
35.
Rizzo
T. R.
Park
Y. D.
Peteanu
L.
, and
Levy
D. H.
, J. Chem. Phys. 83 , 4819 (1985 ).