The long term goal of the Nuclear Receptor Signaling Atlas (NURSA) resides in unraveling the physiological and pathological functions of nuclear receptors (NRs) at the molecular, biochemical and cellular levels. This multi-oriented task requires complementary approaches in order to determine the specific function(s) and precise expression and receptor activity patterns for each individual conventional or orphan receptor. To attain this objective, we have chose to turn to technologies recently made available to engineer bacterial artificial chromosomes (BACs).
References
1.
CopelandN. G., JenkinsN. A., and CourtD. L. (2001). Recombineering: a powerful new tool for mouse functional genomicsNat Rev Genet2, 769–79.
2.
GongS., YangX. W., LiC., and HeintzN. (2002). Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replicationGenome Res12, 1992–8.
3.
LeeE. C., YuD., Martinez de VelascoJ., TessarolloL., SwingD. A., CourtD. L., JenkinsN. A., and CopelandN. G. (2001). A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNAGenomics73, 56–65.
4.
MuyrersJ. P., ZhangY., BuchholzF., and StewartA. F. (2000). RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partnersGenes Dev14, 1971–82.
5.
MuyrersJ. P., ZhangY., and StewartA. F. (2001). Techniques: Recombinogenic engineering–new options for cloning and manipulating DNATrends Biochem Sci26, 325–31.
6.
MuyrersJ. P., ZhangY., TestaG., and StewartA. F. (1999). Rapid modification of bacterial artificial chromosomes by ET-recombinationNucleic Acids Res27, 1555–7.
7.
YangX. W., WynderC., DoughtyM. L., and HeintzN. (1999). BAC-mediated gene-dosage analysis reveals a role for Zipro1 (Ru49/Zfp38) in progenitor cell proliferation in cerebellum and skinNat Genet22, 327–35.
8.
YuD., EllisH. M., LeeE. C., JenkinsN. A., CopelandN. G., and CourtD. L. (2000). An efficient recombination system for chromosome engineering in Escherichia coliProc Natl Acad Sci U S A97, 5978–83.
9.
ZhangY., BuchholzF., MuyrersJ. P., and StewartA. F. (1998). A new logic for DNA engineering using recombination in Escherichia coliNat Genet20, 123–8.
