Viability of the Fungus Metarhizium Anisopliae in Mixture with Agrochemicals

Abstract

Spanish

In attemp to use the entomopathogenic fungus Metarhizium anisopliae as part of an integrated pest management program against the coffee berry borer, Hypothenemus hampei, the commercial (CD) and half commercial doses (1/2CD) of the following substances were tested to determine their effect on the fungus. Insecticides: endosulfan, clorpirifos, pirimifos methyl, fenitrothion, isazofos and neem; fungicides: benomyl, cyproconazol, hexaconazol, triadimefon and copper oxychloride; herbicides: oxyfluorfen, paraquat, glyfosate and glyfosate + terbutilazina; spray additives: Agral, Agrotin, Mezclafix and Mixel; foliar fertilizer: Tottal. The inhibition measured in percentage at 24 h after germination for the CD and 1/2CD was respectively, for the insecticides: pirimifos methyl (98.16 and 57.43%), clorpirifos (97.42 and 58.95%), fenitrothion (100 and 100%) and endosulfan (96.94 and 69.75%); for fungicides: cyproconazol (98.79 and 96.37%), hexaconazol (58.08 and 36.56%) and triamidefon (100 and 95.11%); for herbicides: oxyfluorfen (100 and 100%) and paraquat (99.06 and 98.80%); for spray additives: Mixel (43.06 y 28.93%). The other substances tested had less inhibitory effect (<30%). The fungistatic effect of the pesticides on the fungus decreased 48 h after germination.

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References

Atehortúa

M. M C.

Londoño

Μ. Ε Z.

. 1993. Efecto de algunos agroquímicos en el crecimiento y esporulación del hongo Metarhizium anisopliae. En: Congreso de la Sociedad Colombiana de Entomología, 20°, Cali (Colombia) 13-16 de Julio de 1993. Socolen, Cali. p.21.

Bustillo

A. E.

Villalba

D.A. G.

Chaves

B. C.

1993. Consideraciones sobre el uso de insecticidas químicos en la zona cafetera en el control de la broca del café Hypothenemus hampei. En: Congreso de la Sociedad Colombiana de Entomología, 20°, Cali (Colombia), 13-16 de Julio de 1993. Socolen, Cali. p.152–158.

Cadatal

T. D.

Gabriel

B. P.

1970. Effect of chemical pesticides on the development of fungi pathogenic to some rice insects. Philippine Entomologist v.1 no.5, p.379–395.

Castiñeiras

Calderon

Lopez

1991. Efecto de los biocidas y fertilizantes empleados en el cultivo del plátano en Cuba sobre los hongos entomopatógenos. I. Metarhizium anisopliae. Protección de Plantas (Cuba) v.1 no.1, p.33–42.

Fargues

1992. Traitment mixte des larves de doryphore Leptinotarsa decemlineata Say par des spores du champignon entomopathogene Beauvearia bassina (Bals.) Vuill. et des dos es réduites d'ínsecticide. Phytiatrie-Phytopharmacie (Francia). v.21, p.183–193.

Griffin

D. H.

1994a. Spore development. In: Fungal Physiology. Wiley-Liss, New York. p. 337–374.

Griffin

D. H.

1994b. Nutrient acquisition: Digestion and transport. In: Fungal Physiology. Wiley-Liss, New York. p.158–194.

D. P.

Holdom

D. G.

1994. Effects of pesticides on growth and sporulation of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes). Journal of Invertebrate Pathology (Estados Unidos) v.63 no.2, p.209–211.

McClatchie

G. V.

Moore

Bateman

R. P.

Prior

1994. Effects of temperature on the viability of the conidia of Metarhizium flavoviride in oil formulations. Mycological Research (Reino Unido) v. 99 no. 7, p. 749–756.

10.

Mohamed

A. K. A.

Pratt

J. P.

Nelson

F. R. S.

1987. Compability of Metarhizium anisopliae with chemical pesticides. Mycopathology (Estados Unidos) v.99 no.2, p. 99–105.

11.

Moorhouse

E. R.

Gillespie

A. T.

Sellers

E.K.

Charnley

A.K.

1992. Influence of fungicides and insecticides on the entomogenous fungus Metarhizium anisopliae, a pathogen of the vine weevil, Otiorhynchus sulcatus. Control Science and Technology v.2 no.1, p, 49–58.

12.

Rivera

M A.

Bustillo

A.E. P.

Marin

P. M.

1992. Compatibilidad en mezcla de dos aislamientos de Beauveria bassina (Balsamo) Vuillemin con insecticidas usados en el control químico de la broca del cafe, Hyphotenemus hampei (Ferrari). En: Congreso de la Sociedad Colombiana de Entomología, 20°, Cali (Colombia), 13-16 de julio de 1993. Socolen, Cali, p. 105.

13.

Samuels

K. D. Z.

Pinnock

D. E.

Allsopp

P. G.

1989. The potential of Metarhizium anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes) as a biological control agent of Inopus rubriceps (Macquat) (Diptera: Stratiomyidae). Journal of the Australian Entomogical Society (Australia) v. 28 no.1, p. 69–74.

14.

Vaino

Hokkanen

1990. Side-effects of pesticides on the entomophagous nematode Steinernema feltiae and the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana in the laboratory. In: International Colloquium on Invertebrate Pathology and Microbial Control, S. Adelaide (Australia)., 20-24 August, 1990. Proceedings and Abstracts.