Molecular Techniques as Tools in the Epidemiology Field. Analysis of Dispersion and Reinfestation Possibility by Rhodnius Colombiensis (Hemiptera: Reduviidae)

Abstract

Spanish

Summary

Insects of the genus Rhodnius belonging to the domestic and silvatic habitat were analyzed by molecular techniques to determine the reinfestation possibility of the human dwellings to guide the control programs and epidemiological survellaince. Dendrographs, Fst (0.2421) and Nm (1.05) were generated using informatic packages. These results suggest that genetic flow between the two populations is null, that is to say, they are isolated. However, 4 individuals captured in the houses, were characterized as belonging to the wild population when rDNA was amplified, and also this individuals grouped inside of the wild population cluster, this also suggest a possible migration of wild individuals to domiciliated areas, therefore it is recommended a periodic monitoring of human dwellings to prevent possible reinfestations.

Keywords

rDNA-PCR RAPD Genetic flow Control programs Chagas disease Fst Migration rate

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