Effects of Insecticides on Co₂release by Entomopathogenic Nematodes (Nematoda: Rhabditida) and Development of Their Mutualistic Bacteria

Abstract

Spanish

The present study aimed to evaluate the CO₂ release by infective juveniles (IJs) and the viability of mutualistic bacteria of entomopathogenic nematodes (EPNs) when exposed to synthetic insecticides. Two species of EPNs, Heterorhabditis amazonensis JPM4 and Steinernema carpocapsae All, were tested. The applied insecticides were Vertimec® (abamectin) and Klorpan® (chlorpyrifos). Gas chromatography was employed for CO₂ analysis. The bacteria were isolated and the colony development was evaluated in sterile Petri dishes. Vertimec® triggered greater CO₂ release by the nematodes, associated with changes in their metabolic activity, than Klorpan®. Vertimec® and Klorpan® did not inhibit the bacterial development.

Keywords

Steinernematidae Heterorhabditidae Compatibility Metabolism Abamectin Chlorpyrifos

Get full access to this article

View all access options for this article.

References

ADAMS

B. J.

NGUYEN

K. B.

2002. Taxonomy and systematics. pp.1–34. En: Gaugler

(Ed.). Entomopathogenic nematology. Wallingford. CАВІ. 399 р.

ANDALÓ

MOINO

Jr. MAXIMINIANO

CAMPOS

V. P.

MENDONÇA

L. A.

2011. Influence of temperature and duration of storage on the lipid reserves of entomopathogenic nematodes. Revista Colombiana de Entomología 37 (2): 203–209.

DUTKY

S. R.

THOMPSON

J. V.

CANTWE

G. E.

1964. A technique for the mass propagation of the DD-136 nematode. Journal of Insect Pathology 6 (4): 417–422.

FERREIRA

D. F.

2011. Sisvar: A computer statistical analysis system. Ciência e Agrotecnologia 35: 1039–1042.

GREWAL

P. S.

2001. Anhydrobiotic potential and long-term storage of entomopathogenic nematodes (Rhabditida: Steinernematidae). International Journal for Parasitology 30: 995–1000.

KAYA

H. K.

STOCK

S. P.

1997. Techniques in insect nematology. pp. 281–324. En: Lacey

L. A.

(Ed.). Manual of Tecniques in Insect Pathology. Elsevier. San Diego, California. USA. 281 р.

KOPPENHOFER

A. M

FUZY

E. M.

2008. Effect of the anthranilic diamide insecticide, chlorantraniliprole, on Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) efficacy against white grubs (Coleoptera: Scarabaeidae). Biological Control 45: 93–102.

NEGRISOLI

A. S.

JR BARBOSA

C. R. C.

MOINO

2008. Avaliação da compatibilidade de produtos fitossanitários com nematóides entomopatogênicos (Rhabditida: Steinernematidae, Heterorhabditidae) utilizando o protocolo modificado da IOBC/WPRS. Nematologia Brasileira 32 (2): 111–116.

PARRA

J. R. P.

1998. Criação de insetos para estudos com patógenos. pp. 1015–1037. En: Alves

S. B.

(Ed.). Controle microbiano de insetos. FEALQ. Piracicaba. 1163 p.

10.

SABINO

P. H. S.

SALES

F. S.

GUEVARA

E. J.

MOINO

Jr. FILGUEIRAS

C. C.

2014. Compatibility of entomopathogenic nematodes (Nematoda: Rhabditida) with insecticides used in the tomato crop. Nematoda 1: e03014.

11.

SABINO

P. H. S.

MONIO

Jr. ANDALO

LIMA

L. M. Z.

SALES

F. S.

2015. A method for measuring the concentration of CO₂ released by entomopathogenic nematodes. Revista Colombiana de Entomologia 41 (2): 280–282.

12.

VOSS

ANDALO

NEGRISOLI

A. S.

Jr. BARBOSA NEGRISOLI

C. R.

2009. Manual de técnicas laboratoriais para obtenção, manutenção e caracterização de nematoides entomopatogênicos. Embrapa Trigo. Passo Fundo. 44 p.

13.

S. SABINO

, P.H. MOINO

Jr. ; ANDALÓ

LIMA

L. M. Z.

FILGUEIRAS

C. C.

2017. Effects of insecticides on CO2 release by entomopathogenic nematodes (Nematoda: Rhabditida) and development of their mutualistic bacteria. Revista Colombiana de Entomología 43 (1): 38–40. Enero-Junio 2017. ISSN 0120-0488.