Decrease of Respiration by Glucose (Crabtree Effect) in Rous Sarcoma of Chorioallantoic Membrane. ∗

Summary

Qo₂ values of Rous sarcoma virus-induced chorioallantoic tumors show a depression in oxygen uptake when glucose at 0.1% final concentration is added as substrate. Respiration of normal chorioallantoic membrane was not depressed by addition of glucose. The reaction of the medium with glucose dropped from pH 7.4 to 6.8 with normal tissue and to 6.6 with tumor tissue during 4 hours. Pyruvate as substrate gave increased oxygen uptake with both tumor tissue and normal tissue. These data demonstrate in a virus-induced tumor a metabolic alteration associated with glucose metabolism comparable to that observed in tumors not demonstrated to be of viral etiology.