Studies on the Measurement of Insulin Antibodies in Controlled and Resistant Human Diabetic Patients by Polyacrylamide Gel Electrophoresis ∗

Summary

A new method was developed by using polyacrylamide disc-gel electro-phoresis for the assay of insulin and insulin antibodies in biological materials. By this method we have been able to detect insulin-binding antibodies in 15 out of 16 diabetic patients studied. The antibody titer ranged from 1.6 to 30.0 mU/ml of the serum. From the mobility of the insulin-binding protein (antibody) the molecular weight of this protein (both guinea pig and human and resistant diabetic) was around 150,000.