Production of Heparin Related Mucopolysaccharides by Mammalian Cells in Culture 1

Summary

Radioactive mucopolysaccharides were isolated from HeLa, L, mouse embryo, and rat embryo cells grown in culture medium in the presence of radioactive inorganic sulfate, for 72 hr. The isolated mucopolysaccharides were submitted to mild acid hydrolysis as well as to the action of several mucopolysaccharidases and the degradation products were characterized by their migration in several chromatographic systems and on electrophoresis. It was demonstrated that part of these mucopolysaccharides are chondroitin sulfate A C and part N-sulfated-heparin-like compounds susceptible to hydrolysis by heparinases releasing glucosamine 2. 6-disulfate as the major amino sugar residue.