Isotope Inequilibrium of Glucose Metabolites in Intact Cells and Particlefree Supernatants of Ehrlich Ascites Tumor

Summary

With an enzyme degradative technique, isotope inequilibrium of glucose metabolites was demonstrated in intact cells and particlefree supernatants of Ehr-lich ascites tumor using l-¹⁴C-glucose as tracer. Inequilibrium was found between glucose and glucose-6-phosphate, glucose and fructose-6-phosphate, glucose and 6-phosphogluconate, while glucoses-phosphate and fructose-6-phosphate were found to be in near-equilibrium within the incubation time investigated. Glucose and lactate were found to be in near equilibrium after 8 min in intact cells. Calculations based on the equilibrium levels found, showed that these inequilibria could not be explained by the effects of the pentose cycle.