In Vitro Renal Transport Functions of the Progeny of Rats with Streptozotocin-Induced Diabetes 1

Abstract

The effect of streptozotocin-induced gestational diabetes in the rat on the development of renal tubular functions of progeny was determined using in vitro kidney slice techniques. Accumulation of p-aminohippuric acid (PAH) by renal cortical slices (PAH S/M ratios) from progeny of diabetic mothers (PDMs) was lowest at Day 1 of age and increased from 1 to 28 days of age. For PDMs, PAH S/M ratios were similar to those observed for progeny of control mothers (PCMs) except at 10 days of age when they were significantly lower. Accumulation of α-methylglucoside (MG) by renal cortical slices from PDMs (MG S/M ratios) was similar to that reported for PCMs, except at 10 days of age when accumulation was significantly depressed in PDMs. Accumulation of N-methylnicotinamide (NMN) by renal cortical slices (NMN S/M ratios) from PDMs was similar to that of PCMs. NMN S/M ratios were lowest at birth and increased with age. Accumulation of α-amino-isobutyric acid (AIB) by renal cortical slices (AIB S/M ratios) from PDMs was no different than that from PCMs. AIB S/M ratios were highest at birth and decreased with age. It was concluded that gestational diabetes differentially affected renal tubular transport functions in the progeny.