Abstract
Summary
Poliovirus in tissue culture fluid was treated with dilute formaldehyde and fractionated by ultracentrifugation. Most of the complement-fixing antigen in the active virus preparations could be sedimented at 105,000 x gravity for 2 hours. However, in the formalin-treated samples a soluble antigen appeared which remained in the supernatant fluid. The active virus preparations used were almost completely type specific in their complement-fixing reactions, but after treatment with formaldehyde, cross reactions were found between all types.
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