Abstract
Reactive oxygen species (ROS) are reactive molecules which are produced normally by metabolic reactions. 2,7-Dichlorofluorescein diacetate (DCHF-DA) assay is used to determine intracellular levels of ROS in cancer cells. In the present study, the mechanism and kinetic parameters of this determination are obtained by kinetic Monte Carlo simulation. The values of the rate constants for the suggested mechanism were obtained by simulation. Then, the effect of DCHF-DA concentration on the rate of reaction was studied. According to the results, the product concentration should increase on increasing the DCHF-DA concentration. In addition, the effect of ROS concentration on the rate of reaction was determined.
Keywords
References
1.
Tyrrell R.M.
(2000 ) Meth. Enzymol. , 319 , 290 –296 .
2.
Dabrowski A.
,
Boguslowicz C.
,
Dabrowska M.
,
Tribillo I.
, and
Gabryelewicz A.
(2000 ) Pancreas , 21 , 376 –384 .
3.
Curtin J.F.
,
Donovan M.
, and
Cotter T.G.
(2002 ) J. Immunol. Methods , 265 , 49 –72 .
4.
Doreswamy K.
(2005 ) Toxicology , 206 , 169 –178 .
5.
Lieber C.S.
(2004 ) Alcohol , 34 , 9 –19 .
6.
Behl C.
(2005 ) Subcell Biochem. , 38 , 65 –78 .
7.
Sanz A.
,
Caro P.
, and
Barja G.
(2004 ) J. Bioenerg. Biomembr. , 36 , 545 –552 .
8.
Tateyama M.
,
Takeda A.
,
Onodera Y.
,
Matsuzaki M.
,
Hasegawa T.
,
Nunomura A.
,
Hirai K.
,
Perry G.
,
Smith M.A.
, and
Itoyama Y.
(2003 ) Acta Neuropathol. (Berl.) , 105 , 581 –585 .
9.
Kikuchi A.
,
Takeda A.
,
Onodera H.
,
Kimpara T.
,
Hisanaga K.
,
Sato N.
,
Nunomura A.
,
Castellani R.J.
,
Perry G.
,
Smith M.A.
, and
Itoyama Y.
(2002 ) Neurobiol. Dis. , 9 , 244 –248 .
10.
Sun A.Y.
, and
Chen Y.M.
(1998 ) J. Biomed. Sci. , 5 , 401 –414 .
11.
Keston A.S.
, and
Brandt R.
(1965 ) Anal. Biochem. , 11 , 1 –5 .
12.
Balasubramanyam M.
,
Koteswari A.A.
,
Kumar R.S.
,
Monickaraj S.F.
,
Maheswari J.U.
, and
Mohan V.
(2003 ) J. Biosci. , 28 , 715 –721 .
13.
Crow J.P.
(1997 ) Biol. Chem. , 1 , 145 –157 .
14.
Babo S.
, and
Charbonneau M.
(1994 ) Toxicol. Meth. , 4 , 224 –233 .
15.
Qian Z.J.
,
Jung W.K.
,
Byun H.G.
, and
Kim S.K.
(2008 ) Bioresource Technol. , 99 , 3365 –3371 .
16.
Loetchutinat C.
,
Kothan S.
,
Dechsupa S.
,
Meesungnoen J.
,
Jay-Gerin J.
, and
Mankhetkorn S.
(2005 ) Radiat. Phys. Chem. , 72 , 323 –331 .
17.
Burkitt M.J.
, and
Wardman P.
(2001 ) Biochem. Biophys. Res. Commun. , 282 , 329 –333 .
18.
Ohashi T.
,
Mizutani A.
,
Murakami A.
,
Kojo S.
,
Ishii T.
, and
Taketani S.
(2002 ) FEBS Lett. , 511 , 21 –27 .
19.
Gillespie D.T.
(1976 ) J. Comp. Phys. , 2 , 403 –434 .
20.IBM, CSK Chemical Kinetics Simulator 1.01, IBM Almaden Research Center ; IBM Corporation (1995 ).
21.
Zarzycki P.
(2007 ) J. Colloid Interface Sci. , 315 , 54 –62 .
22.
Liau L.
, and
Lin Ch.Y.
(2011 ) Colloids Surf. A , 388 , 70 –76 .
23.
Rojas M.I.
,
Gimenez M.C.
, and
Leiva E.P.M.
(2005 ) Surf. Sci. , 581 , 109 –114 .