Experimental verification of depolarization effects in bioelectrical impedance measurement

The electrode polarization effects on bioelectrical impedance measurement at low-frequency cannot be ignored. In this paper, the bioelectrical data of mice livers are measured to specify the polarization effects on the bio-impedance measurement data. We firstly introduce the measurement system and methodology. Using the depolarization method, the corrected results are obtained. Besides, the specific effects of electrode polarization on bio-impedance measurement results are investigated using comparative analysis of the previous and posterior correction results from dielectric spectroscopy, Cole-Cole plot, conductivity and spectroscopy of dissipation tangent. Experimental results show that electrode polarization has a significant influence on the characteristic parameters of mouse liver tissues. To be specific, we see a low-frequency limit resistance R₀ increase by 19.29%, a reactance peak X_P increase by 8.50%, a low-frequency limit conductivity K_l decrease by 17.65% and a dissipation peak tangent decrease by 160%.