Journal of Cellular Biotechnology

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Research article
Available accessResearch articleFirst published February, 2026pp. 3-9
The antimicrobial activity of single and combined nanoparticles on bacterial growth
Ali Bin ThaniORCIDKaltham Moosa Jaafar HazeemAsala Ali Abdalwali Ebrahim
Abstract
In nanotechnology, nanoparticles (NPs) are small particles 1–100 nm in size that can occur in nature or be synthesized in a variety of ways, such as through biological or chemical processes, and come in many shapes and sizes. Based on their chemical composition, nanoparticles are classified into four categories: carbon-based NPs, organic/inorganic NPs, and composite NPs. NPs gained importance with the development of science and biotechnology due to their chemical and physical properties, which make them useful in many fields such as medicine, environmental protection, and agriculture. Furthermore, NPs exhibit antimicrobial activity against a variety of bacteria, including both gram-negative and gram-positive bacteria as well as multidrug-resistant bacteria. The results of this study showed a decrease in growth for both gram-negative bacteria E. coli and gram-positive bacteria S. aureus when exposed to increasing concentrations of copper and nickel oxide NPs. For the combined (0.8 M sodium thiosulfate, 0.02 M sodium caprylate, and 2M oxalic acid) NPs the results showed larger inhibition zone in the bacteria at the higher concentration of NPs (30 mg/mL) when applied in a well which means greater diffusion of NPs in the agar.
Research article
Available accessResearch articleFirst published February, 2026pp. 10-17
Effect of chronic consumption of coffee on complete blood count and histology of the liver
Solomon Matthias GamdeORCIDEne FrancisJohnbull Ogboi
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Abstract
Background
Coffee is one of the most popular beverages in the world. Most people drink a cup of coffee to start and wrap up their daily tasks. Bioactive compounds of coffee such as caffeine, diterpenes, and chlorogenic acids are known to stimulate physiological processes. Students also ingest coffee in an attempt to stay up late reading. We aimed to determine the effect of chronic consumption of coffee on complete blood count and liver histology in Wistar rats.
Methods
Wistar rats were divided into control Group 1, Group 2, Group 3, and Group 4 and were fed with water and feed, 100 mg/kg body weight, 200 mg kg/body weight, and 300 m/kg body weight of coffee, respectively, for 3 weeks. Blood samples were analyzed for hematological parameters, and liver tissues were histologically examined for structural changes.
Result
There was a dose-dependent increase in the body weight of coffee-treated animals. The hemoglobin, pack cell volume, and red blood cell count also significantly increased in animals administered with coffee compared to controls. However, chronic ingestion induces apoptotic changes in the liver.
Conclusion
Our data suggest that coffee has a positive effect on hematological indices. However, chronic consumption is injurious to the liver in rats.
Research article
Available accessResearch articleFirst published February, 2026pp. 18-25
Microrheological responses of red blood cells to hydrogen sulfide in normotensive and hypertensive individuals: Analysis of intracellular signaling pathways
A. MuravyovI. TikhomirovaA. Priezzhev
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Abstract
Background
It is now known regulatory effect of gaseous mediators (NO and H₂S) in many bodily functions. However, detailed data on the regulatory role of each of these gasotransmitters are still not sufficiently elucidated.
Objective
The aim of this study was to estimate RBC microrheological changes under the influence of H₂S donor in normotensive and hypertensive (AH) persons and the signaling pathways associated with these effects.
Methods
RBCs were incubated with: NaHS (H₂S donor), ODQ, methylene blue, L-NAME, and SNP (NO donor). RBC deformability (RBCD) and aggregation (RBCA) were evaluated and compared with the corresponding control cell suspension.
Results
There was a difference in RBCD and RBCA between healthy individuals and AH patients, 6 and 58%, respectively. NaHS increased RBCD in both groups of individuals and significantly reduced RBCA. These positive effects of NaHS were eliminated by inhibition NO signaling pathway. A greater microrheological effect was observed with the combined action of NO and NaHS.
Conclusion
NO and H₂S donors demonstrated cross-talk and caused a greater change in microrheological characteristics than their separate effects. The obtained data indicate that hydrogen sulfide, in microrheological responses to its exposure, may use the elements of the NO-associated signaling pathway.
Research article
Open accessResearch articleFirst published February, 2026pp. 26-29
The cannabinoid type 2 receptor agonist,HU308,reduces cytokine secretion in LPS-stimulated RAW 264.7 macrophages
Stefan HallKayle DicksonORCIDJuan Zhou
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Abstract
Introduction
Inflammatory mediators produced by macrophages play a key role in the immune response to sepsis. Agonism of the cannabinoid receptor two represents a promising anti-inflammatory strategy that can modulate macrophage activation in this context. In this article, we highlight the use of the synthetic cannabinoid HU-308 as an anti-inflammatory therapeutic agent in an in vitro model of macrophage inflammation.
Methods
To this end, RAW 264.7 murine macrophages were stimulated with lipopolysaccharide and their production of inflammatory mediators was evaluated using enzyme-linked immunosorbant assays. Further, we evaluated macrophage viability with HU-308 treatment and confirmed the expression of the cannabinoid receptor 2.
Results
We identified a dose-related reduction in the secretion of IL-6, TNF, and CXCL8.
Conclusion
HU-308 is a promising candidate for reducing inflammation secondary to macrophage activation, indicating the value of additional research on this topic.
Review article
Available accessReview articleFirst published February, 2026pp. 30-36
Bioactive nanoparticles from plants: A novel strategy for cancer therapy
DV Surya PrakashAshima KathuriaORCIDAvinash Singh
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Abstract
Studies on nanotechnology in cancer treatment surpass drug delivery, advancing towards the development of novel therapeutics that can only be achieved through the inherent properties of nanomaterials. In contemporary research and cancer treatment, nanotechnology has emerged as one of the most inventive, quick, and flexible fields. Due to its non-specificity and detrimental effects on normal cells, conventional chemotherapy has limitations and is still a major global health concern. Nanoparticles have become a promising approach for fighting cancer due to their ability to precisely target cancer cells and deliver therapeutic substances to them. Unique characteristics based on dimensions, distribution, and forms are revealed by nanomaterials. The nanoparticles are enhanced anticancer agents due to the coating of phytoconstituents as functional groups to cancer cells for increase the rate of apoptosis. The potential of biosynthesized nanoparticles for cancer treatments is covered in this paper. An effective method with anti-cancer properties, biosynthesized nanoparticles can halt the growth of tumors and cause cancer cells to undergo apoptosis. They could be set up to target particular cancer cells with drugs or other treatments. Notwithstanding its promise, nanoparticles include inconsistent synthesis and characterization, possible toxicity, and effectiveness against different types of cancer.
Review article
Available accessReview articleFirst published February, 2026pp. 37-46
Epigenetic modifications and gene expression regulation in testosterone-mediated prostate cancer: A systematic review
Vinod Kumar SinghORCIDJigar HariaPothu Usha Kiran
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Abstract
Background
Prostate cancer is known to be caused by the androgen receptor (AR) axis and testosterone, emerging evidence highlights the significance of epigenetic modifications in mediating androgen-dependent tumorigenesis and therapy resistance.
Methodology
The literature search was conducted in Scopus, PubMed, and Google Scholar following PRISMA guidelines. Case-control, clinical and preclinical studies, and human clinical trials analyzed epigenetic modifications in testosterone-mediated prostate cancer were included. Studies that did not analyze epigenetic alterations in prostate cancer or focused on other cancer types were excluded.
Result
Prostate cancer is largely caused by DNA methylation, which controls gene expression. Genes are silenced when CpG islands are hypermethylated, and genomic instability is exacerbated by hypomethylation. Emerging therapeutic strategies, such as DNMT inhibitors and combination therapies with AR pathway inhibitors, are being explored to target aberrant DNA methylation in prostate cancer treatment. Prostate cancer development and androgen receptor signaling are influenced by chromatin remodeling and gene regulation, both of which are significantly impacted by histone changes.
Conclusion
Epigenetic dysregulation drives prostate cancer progression by silencing tumor suppressors, activating oncogenes, and promoting therapy resistance. These changes have potential as therapeutic targets for better patient clinical care.
Review article
Available accessReview articleFirst published February, 2026pp. 47-60
Insights into the nucleolar dysfunction and associated genetic disorders
Pothu UshakiranORCIDJigar HariaVinod Kumar SinghORCID
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Abstract
The nucleolus, a dynamic and membrane-less nuclear subcompartment, plays a central role in maintaining cellular homeostasis. Traditionally recognized as the site of ribosomal RNA (rRNA) synthesis and ribosome assembly, the nucleolus is now known to participate in diverse cellular processes, including cell-cycle regulation, DNA-damage response, and stress signaling. Structurally organized into three main components—the fibrillar center (FC), dense fibrillar component (DFC), and granular component (GC)—it undergoes rapid remodeling under physiological and pathological conditions.

Dysfunction of the nucleolus has been increasingly linked to a group of genetic disorders collectively termed ribosomopathies, characterized by defective ribosome biogenesis and function. Mutations affecting nucleolar proteins or rRNA-processing factors can disrupt ribosomal output, activate the p53 pathway, and result in tissue-specific abnormalities, hematological disorders, and heightened cancer susceptibility. Notable ribosomopathies include Diamond–Blackfan anemia, Treacher Collins syndrome, and Dyskeratosis congenita.

This review provides a comprehensive overview of the structural and functional aspects of the nucleolus, explores its role in genetic-disease mechanisms, and highlights current advances in understanding nucleolar stress and its downstream effects. We also discuss how emerging insights into nucleolar biology are revealing novel diagnostic and therapeutic strategies. Given the centrality of the nucleolus in cellular physiology, elucidating its dysfunction may significantly improve our understanding of a wide spectrum of human diseases.
Book review
Available accessBook reviewFirst published February, 2026pp. 61-62
Harnessing cyanobacteria as solar cell factories: A book review of cyanobacteria in biotechnology: Applications and quantitative perspectives
Jomar L. AbanORCID
Retraction
Available accessRetractionFirst published February, 2026pp. 63-63
Retraction notice

Volume 12, Issue 1, February 2026